目的 对维药骆驼刺Alhagi sparsifolia水溶性化学成分进行研究。方法 采用多种色谱技术进行分离纯化，通过理化性质、波谱数据和X单晶衍射鉴定化合物结构，测试各化合物对人宫颈癌HeLa细胞的细胞毒性。结果 从骆驼刺地上部分分离得到21个水溶性化合物，分别鉴定为毒豆甲酮（1）、毒豆乙酮（2）、callyspongidipeptideA（3）、环(脯氨酸-亮氨酸)（4）、对羟基苯甲醇（5）、D-脯氨酸-D-缬氨酸（6）、环(脯氨酸-亮氨酸)二肽（7）、布卢姆醇A（8）、(3S,4R)-3,4-二羟基-3-甲基二氢呋喃-2(3H)-酮（9）、isololiolide（10）、dehydrovomifoliol（11）、N-苯乙基甲酰胺（12）、3-hydroxy-β-damascone（13）、3,4-二羟基-3-甲基二氢呋喃-2(3H)-酮（14）、环(丙氨酸-脯氨酸)（15）、脯氨酸（16）、布卢姆醇B（17）、蚱蜢酮（18）、N-(对羟基苯乙基)甲酰胺（19）、N-乙酰基酪胺（20）、(＋)-丁香脂素-O-β-D-葡萄糖苷（21）；并对化合物1～21进行了抗宫颈癌活性筛选。结论 化合物1～7、9～20为首次从骆驼刺属植物中分离得到，且化合物4、7、15表现出潜在的抗肿瘤活性，半数抑制浓度（median inhibition concentration，IC₅₀）分别为（29.09±0.99）、（31.51±1.89）、（28.25±0.65）μmol/L。

Objective To study the water soluble chemical constituents of Alhagi sparsifolia. Methods A variety of chromatographic techniques were used to separate and purify, and the structure of the compounds was identified by physicochemical properties, spectral data and X-single crystal diffraction, and the cytotoxicity of each compound to HeLa cells was tested. Results Twenty-one water-soluble compounds were isolated from the above-ground parts of A. sparsifolia after the process of extraction and were identified as drummondone A (1), drummondone B (2), callyspongidipeptideA (3), cyclo(Pro-Leu) (4), 4-(hydroxymethyl)phenol (5), cyclo(D)-Pro-(D)-Val (6), cyclo(L-pro-L-Leu) (7), blumenol A (8), (3S,4R)-3,4-dihydroxy-3-methyldihydrofuran-2(3H)-one (9), Isololiolide (10), dehydrovomifoliol (11), N-phenethylformamide (12), 3-hydroxy-β-damascone (13), 3,4-dihydroxy-3-methyldihydrofuran-2(3H)-one (14), cyclo(L-Pro-L-Ala) (15), proline (16), blumenol B (17), grasshopper ketone (18), N-(p-hydroxyphenethyl) (19), N-acetyltyramine (20), (＋)-syringaresinol O-β-D-glucopyranoside (21), and compounds 1―21 were screened for anti-cervical cancer activity. Conclusion Compounds 1—7, 9—20 were the first known compounds within the genus, and compounds 4, 7 and 15 showed potential antitumor activity with median inhibition concentration (IC₅₀) of (29.09±0.99), (31.51±1.89) and (28.25±0.65) μmol/L, respectively.

R284.1

新疆维吾尔自治区重点研发计划项目（2022B03018-4）