目的 建立HPLC指纹图谱与一测多评（quantitative analysis of multi-components with a single-marker，QAMS）结合的方法同时检测顶羽菊Rhaponticum repens药材中新绿原酸、绿原酸、隐绿原酸、1,5-二咖啡酰奎宁酸、异绿原酸C、芹菜素、高车前素的含量，并建立不同产地顶羽菊药材的综合质量评价模型，为顶羽菊药材的整体质量评价提供参考。方法 采用HPLC法建立不同产地16批顶羽菊药材的指纹图谱，利用化学计量学模式聚类分析（cluster analysis，CA）和正交偏最小二乘判别分析（orthogonal partial least squares-discrimination analysis，OPLS-DA）对采集的指纹图谱信息进行分析，同时运用加权逼近理想解排序法（technique for order preference by similarity to ideal solution，TOPSIS）、加权秩和比法（rank sum ratio，RSR）及两者模糊联合的方法构建评价模型；以绿原酸为内标，确定新绿原酸、隐绿原酸、1,5-二咖啡酰奎宁酸、异绿原酸C、芹菜素、高车前素的相对校正因子并计算其含量，建立QAMS法。结果 建立的顶羽菊药材指纹图谱共标定23个共有峰，指认出其中7个峰。化学计量学模式研究显示不同产地顶羽菊存在明显差异。通过OPLS-DA法筛选出10个主要的差异性成分。构建的熵权TOPSIS法、RSR法以及两者模糊联合的综合质量评价模型，对不同产地顶羽菊药材的质量评价排序结果较为一致。以绿原酸为内标建立的QAMS和外标法含量测定结果无显著性差异（P＞0.05）。结论 所建立的HPLC指纹图谱结合QAMS法简便可靠，重复性好；建立的综合质量评价模型的分析结果全面客观，可用于顶羽菊药材整体质量的综合评价。

Objective To establish a method combining high performance liquid chromatography (HPLC) fingerprint with quantitative analysis of multi-components with a single marker (QAMS) for simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,5-dicaffeoyl quinic acid, isochlorogenic acid C, apigenin and hispidulin in Acroptilon repens. The comprehensive quality evaluation model of A. repens medicinal materials from different producing areas was established to provide reference for the overall quality evaluation. Methods HPLC method was used to determine the fingerprints of 16 batches of A. repens from different origins, and the information of fingerprinting spectrum was analyzed by stoichiometric models such as cluster analysis (CA) and orthogonal partial least-squares discrimination analysis (OPLS-DA). At the same time, the technique for order preference by similarity to ideal solution (TOPSIS), the weighted rank sum ratio (RSR) and the fuzzy combination of the two methods to construct the evaluation model. With chlorogenic acid as the internal standard, the relative correction factors (RCF) of neochlorogenic acid, cryptochlorogenic acid, 1,5-dicaffeoyl quinic acid, isochlorogenic acid C, apigenin and hispidulin were determined and their contents were calculated to establish QAMS method. Results A total of 23 common peaks were calibrated and seven of them were identified by the established fingerprint of A. repens. The study of stoichiometric model showed that there were obvious differences among different producing areas of A. repens. A total of ten differential components were selected by OPLS-DA method. The comprehensive quality evaluation model of entropy weight TOPSIS method, RSR method and their fuzzy combination showed the consistent quality evaluation ranking results of A. repens medicinal materials from different producing areas. There was no significant difference between the results of QAMS with chlorogenic acid as internal standard and the results of external standard method (P > 0.05). Conclusion The established HPLC fingerprint combined with QAMS method is simple, reliable and has good repeatability. The results of the comprehensive quality evaluation model established are comprehensive and objective, which can be used for the comprehensive evaluation of the overall quality of A. repens.

R286.2

新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目（KY2021095）;新疆维吾尔自治区自然科学基金（2022D01A304）