目的 采用指纹图谱与化学模式识别方法，以香豆素为指标，探讨不同种质白芷药材Angelicae Dahuricae Radix发育过程中有效成分的含量变化规律，评价不同种质白芷的质量，为白芷药材合理采收和质量控制提供参考和依据。方法 采用HPLC法测定5个种质白芷不同发育时期香豆素类成分含量，建立不同种质白芷药材化学指纹图谱，结合相似度分析、聚类分析、主成分分析对不同种质白芷进行比较和质量评价。结果 建立了不同种质白芷药材的化学指纹图谱，共标定了19个共有峰，35批白芷相似度均在0.871～0.995；在各种质白芷发育过程中，总香豆素含量呈现先增加后降低的趋势，在7月中旬达到最高；5个种质白芷不同采收期样本的主成分分析结果显示：5个种质白芷的综合评分均在7月中旬最高；5个种质白芷7月中旬样本的主成分分析的综合评分结果：川白芷＞南川白芷＞禹白芷＞祁白芷＞亳白芷，且川白芷的总香豆素含量及欧前胡素含量显著高于其余种质。结论 白芷香豆素类成分组成与含量既取决于种质又受发育程度的影响，各种质白芷最佳采收期为7月中旬，以川白芷质量最佳。

Objective The fingerprint and chemical pattern recognition methods were used to evaluate the quality of different germplasm of Baizhi (Angelicae Dahuricae Radix, ADR) using coumarin as the index, and the change of the content of active ingredients in the development process of different germplasm of ADR was discussed, which provided a reference and basis for the rational harvesting and quality control of ADR.Methods HPLC method was used to determine the content of coumarins in different developmental stages of five germplasm species, and the chemical fingerprints of different germplasm of ADR were established, and the similarity analysis, cluster analysis and principal component analysis were combined to compare and evaluate the quality of different germplasm of ADR. Results The chemical fingerprints of different germplasm of ADR were established, a total of 19 common peaks were calibrated, and the similarity of 35 batches was 0.871—0.995; during the development of various germplasm of ADR, the total coumarin content showed a trend of first increasing and then decreasing, reaching the highest in mid-July. The principal component analysis results of samples from different harvest stages of five germplasm of ADR showed that the comprehensive scores of the five germplasm of ADR were highest in mid-July. The principal component analysis and comprehensive scoring results of five germplasm of ADR samples in mid-July showed that Sichuan Baizhi＞Nanchuan Baizhi＞Yubaizhi＞Qibaizhi＞Bobaizhi, and the total coumarin content and imperatorin content of Sichuan Baizhi were significantly higher than those of other germplasm. Conclusion The composition and content of coumarin components were determined by both germplasm and development degree, and under the experimental conditions, the best harvest period of various species was mid-July, and the quality of Sichuan Baizhi was the best.

R286.2

四川省科技计划重点研发项目（2020YFN0152，2023YFS0338）；四川省科技厅重点研发计划（重大科技专项）社会发展领域重点研发项目（2022YFS0582）；国家中医药管理局中医药创新团队及人才支持计划项目（ZYYCXTD-D-202209）；成都中医药大学杏林学者青基人才专项（QJRC2022034）