目的 基于文献计量学对国内外中药DNA条形码的相关研究进行分析，进而了解此领域研究现状，揭示研究热点和趋势以及提供参考和指导。方法 收集整理Web of Science核心合集数据库和中国知网（CNKI）数据库中2000年至2023年12月31日中药DNA条形码的相关研究文献，使用CiteSpace和VOSviewer对发文量、作者、机构、关键词共现网络等进行可视化分析。结果 中药DNA条形码相关研究仍处于高水平研究阶段，预计未来几年该领域研究在国内外将继续保持较高关注度。中、英文文献均形成以陈士林院士、姚辉、宋经元、孙伟等为核心的研究团队。中国中医科学院中药研究所、中国医学科学院北京协和医学院分别为中、英文文献发文量最多的机构。高效通用的植物条形码的开发、基于DNA条形码和叶绿体基因组测序的中药物种鉴定、种质资源保护、质量控制及中成药生物组分鉴定研究可能是近年的研究热点。结论 广大科研工作者未来应致力开发高效通用的植物条形码，推动基于DNA条形码的中药种质资源鉴定及遗传多样性分析、药用植物全基因组和细胞器基因组测序，将DNA条形码与理化分析方法相结合以实现药材质量综合评价，进一步将DNA条形码技术引入中药全产业链，实现全程监管和可追溯。

Objective To analyze the related research on DNA barcoding in the field of Chinese materia medica (CMM) based on bibliometrics, to understand the current research status, reveal research hotspots and trends, and provide references and guidance. Methods The relevant research literature on DNA barcoding in CMM was collected and organized from the Web of Science Core Collection database and the China National Knowledge Infrastructure database from 2000 to December 31, 2023. CiteSpace and VOSviewer were used to visualize and analyze the number of publications, authors, institutions, and keyword co-occurrence networks. Results Currently, DNA barcoding research in CMM remains at a high level of research stage, and it is expected that the research will continue to maintain a significant attention domestically and internationally in the coming years. Both Chinese and English literature show core research teams led by academician Shilin Chen, Professors Hui Yao, Jingyuan Song, and Wei Sun. The Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, and Peking Union Medical College, Chinese Academy of Medical Sciences, are the institutions with the highest publication volume in both Chinese and English literature. The development of efficient and universal plant barcodes, identification of CMM species based on DNA barcoding and chloroplast genome sequencing, germplasm resource protection, quality control, and research on the identification of bioactive components of Chinese patent medicines may be recent research hotspots. Conclusion In the future, researchers should focus on developing efficient and universal plant barcodes, promoting the identification and genetic diversity analysis of CMM germplasm resources based on DNA barcoding, advancing whole-genome sequencing of medicinal plants and organelle genomes, integrating DNA barcoding with physicochemical analysis methods to achieve comprehensive evaluation of medicinal material quality, further integrating DNA barcoding technology into the entire CMM industry chain to achieve full supervision and traceability.

R282.1

国家自然科学基金项目（82174069）；湖南省重点领域研发计划（2023SK2046）；湖南省自然科学基金项目（2023JJ60474）；湖南省卫生健康高层次人才重大科研专项（R2023139）；长沙市自然科学基金项目（kq2208191）；湖南创新型省份建设专项（2023NK4110）；湖南省科技创新计划项目（2023NK4144）；湖南省研究生创新项目（CX20230829）；校级创新项目（2023CX145，2023CX105，202210541071，S202210541077）