目的 研究老挝厚叶沉香Aquilaria crassna植物所产沉香的化学成分及其抑制一氧化氮作用。方法 采用硅胶、Sephadex LH-20凝胶、高效液相色谱等多种色谱技术分离纯化得到单体化合物；通过质谱、核磁共振等谱学方法并结合理化性质鉴定其结构；采用脂多糖诱导的小鼠单核巨噬细胞RAW264.7模型测试化合物抑制一氧化氮产生的抗炎活性。结果 从老挝沉香的乙醇提取物中分离鉴定3个化合物，分别是厚叶沉香醇（1）、8-氯-6-羟基-2-(2-苯乙基)色酮（2）和8-氯-6-羟基-2-[2-(4-甲氧基苯)乙基]色酮（3）。化合物1对脂多糖诱导的小鼠单核巨噬细胞RAW264.7产生一氧化氮具有抑制作用，其半数抑制浓度值为（47.53±2.14）μmol/L。结论 化合物1为新化合物，命名为厚叶沉香醇，是从沉香中首个发现的由2-(2-苯乙基)色酮和木脂素形成的杂合物，且对脂多糖诱导的RAW264.7细胞株产生一氧化氮呈现抑制活性。

Objective Tto investigate the chemical constituents from Aquilaria crassna in Laos and their inhibition of nitric oxide production activity. Methods Various chromatographic techniques, including silica gel, Sephadex LH-20 gel, high performance liquid chromatography (HPLC) were used for the separation and purification to gain monomer compounds. The structures of these compounds were by mass spectrometry (MS) and nuclear magnetic resonance (NMR) and other spectroscopic methods combined with physical and chemical properties. And lipopolysaccharide (LPS)-induced mouse mononuclear macrophage RAW264.7 model was used to test the anti-inflammatory activity of the compound on nitric oxide (NO) production. Results Three 2-(2-phenylethyl) chromones derivertives were isolated from ethanol extract of A. crassna and identified as aquicrassnol (1), 8-chloro-6-hydroxy-2-(2-phenylethy) chromone (2), 8-chloro-6-hydroxy-2-[2-(4-methoxyphenyl) ethyl]chromone (3). In vitro anti-inflammatory assay results showed that compound 1 inhibit the NO production in mouse mononuclear macrophages RAW264.7 induced by LPS with half maximal inhibitory concentration (IC₅₀) value of (47.53 ± 2.14) μmol/L. Conclusion Compound 1, a new compound named aquicrassnol, was the first heteropolymer composed of 2-(2-phenylethyl) chromone and lignan from Chenxiang (Aquilariae Lignum Resinatum), and showed inhibitory activity on the production of NO in RAW264.7 cell line induced by LPS.

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海南省自然科学基金青年基金资助项目（324QN317）；海南省重大科技计划项目（ZDKJ2021031）；财政部和农业农村部国家现代农业产业技术体系专项资金（CARS-21）