链霉菌属微生物是生产抗生素的优势菌群，也是一些维生素和酶的重要来源，具有广阔的开发前景；然而，链霉菌代谢网络和调控机制的复杂性，及大量基因簇的沉默限制了其次级代谢产物的高效合成。快速发展的代谢工程技术为人为干预、调控链霉菌代谢途径或代谢通量提供了新的手段，并已成为实现链霉菌源目标产物高产和成药性天然产物挖掘的重要途径。通过对以强化链霉菌次级代谢产物合成为目标的非靶向和靶向调控技术的相关研究进展进行综述，为链霉菌次级代谢产物的高效挖掘及生物合成过程强化提供参考。

Microorganisms of the Streptomyces genus are a dominant group of microorganisms capable of producing antibiotics, and are also an important source of some vitamins and enzymes, which have a broad development prospect. However, the complexity of metabolic network and regulatory mechanisms and the silence of a large number of gene clusters in Streptomyces have strictly limited the efficiency of secondary metabolites biosynthesis. The rapid development of metabolic engineering technology provides a new means for human intervention and regulation of Streptomyces metabolic pathway or metabolic flux, and has become an important way to achieve high yield of Streptomyces source target products and the extraction of chemogenic natural products. Based on this, this article mainly focused on the progress in non-targeted and targeted metabolic regulation techniques aimed at enhancing of secondary metabolites biosynthesis of Streptomyces, which can provide a reference for efficient exploration and biosynthesis process enhancement of the secondary metabolites of Streptomyces.

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国家自然科学基金资助项目（81973211/C0033375）;湖南中医药大学研究生创新课题（校行研字 [2022] 20号-87）;湖南中医药大学“十四五”重点学科-生物工程学科（校行发规字[2023] 2号）