目的 采用超高效液相色谱-四级杆-飞行时间质谱联用（UPLC-Q-TOF-MS/MS）技术比较板蓝根Isatidis Radix和大青叶Isatidis Folium的化学成分。方法 采用Accliam 120 C₁₈色谱柱（250 mm×4.6 mm，5 μm；Thermo Fisher），以0.1%甲酸的乙腈溶液-0.1%甲酸的水溶液为流动相，梯度洗脱，体积流量为0.3 mL/min，柱温为40 ℃，采用电喷雾离子源（ESI），在正、负离子模式下进行数据采集，以PeakView 1.2质谱分析软件分析数据。结果 通过化合物的保留时间、精确质荷比、二级质谱裂解碎片等信息，结合对照品、参考文献和Massbank等数据库共鉴定或指认出板蓝根和大青叶的71个共有成分，主要包括生物碱、黄酮、木脂素、含硫化合物、有机酸等类化合物；板蓝根特有成分6个，主要包括含硫化合物类等；大青叶特有成分2个，均为生物碱类。结论 该方法能够系统、快速地比较分析板蓝根和大青叶的化学成分，为其质量控制和药效物质基础研究提供了重要依据。

Objective To compare the chemical constituents of Banlangen (Isatidis Radix) and Daqingye (Isatidis Folium) by UPLC-Q-TOF-MS/MS technology. Methods The liquid phase separation was performed on Accliam 120 C₁₈ (Thermo Fisher, 250 mm×4.6 mm, 5 μm) column, the mobile phases were 0.1% formic acid acetonitrile and 0.1% formic acid solution with gradient elution, flow rate was 0.3 mL/min, column temperature was 40 ℃. Mass spectrometry was performed using electrospray ion (ESI) source under positive and negative ion mode. Retention time, precise mass charge ratio, secondary mass spectrometry and other mass spectrometry data were analyzed using PeakView® 1.2 software (AB Sciex, Foster City, CA, USA) based on reference materials, literature data and Massbank database. Results A total of 71 compounds were identified and speculated in Isatidis Radix and Isatidis Folium, including alkaloids, flavonoids, lignans, sulfur compounds and organic acids. Six compounds were identified and speculated only in Isatidis Radix, including sulfur compounds, and two compounds were identified and speculated only in Isatidis Folium which both belongs to alkaloids. Conclusion The established method can systematically and quickly compare the chemical constituents of Isatidis Radix and Isatidis Folium, which could provide an important basis for the research of quality control and pharmacodynamic material basis.

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中国博士后科学基金资助项目（2021M700913）；广州市科学技术协会青年科技人才托举工程项目（QT20230101122）