目的 对艾绒的化学成分进行研究。方法 采用硅胶、ODS、MCI、Sephadex LH-20和prep HPLC等现代色谱技术对其化学成分进行分离纯化，利用MS、NMR等现代波谱技术鉴定单体化合物结构；采用脂多糖（lipopolysaccharide，LPS）诱导的小鼠巨噬细胞RAW264.7炎症模型，开展新化合物的抗炎活性评价。结果 从艾绒醋酸乙酯提取物中分离得到13个化合物，分别鉴定为 (4S,5S)-5-[(R)-5'-hydroxy-2'-methyl-3'-oxocyclopent-1'-en-1'-yl]-3-methylene-4-(3-oxobutyl)dihydrofuran-2(3H)-one（1）、iso-seco-tanapartholide（2）、3-methoxy-tanapartholide（3）、3-acetyl-iso-seco-tanapartholide（4）、甲基条叶蓟素（5）、圣草酚（6）、泽兰黄素（7）、半齿泽兰素（8）、茵陈色原酮（9）、矢车菊黄素（10）、柚皮素（11）、高圣草酚（12）、异泽兰黄素（13）。化合物1能够抑制LPS诱导的RAW264.7细胞中NO的生成量，半数抑制浓度（median inhibition concentration，IC₅₀）值为（31.15±2.30）μmol/L。结论 化合物1为新化合物，并命名为1R-长叶艾菊内酯，化合物4为首次从菊科植物中分离得到；化合物1具有一定的抗炎活性。

Objective To investigate the chemical constituents of moxa floss. Methods The chemical constituents were isolated and purified by silica gel, ODS, MCI, Sephadex LH-20 and prep HPLC, and their structures were elucidated using MS and NMR. Meanwhile, the Griess method was used to evaluate the anti-inflammatory activity by testing the inflammatory factor release model induced by lipopolysaccharide (LPS) in mouse macrophage RAW264.7 cells. Results A total of 13 compounds were isolated from the ethyl acetate extract of moxa floss and identified as (4S,5S)-5-((R)-5'-hydroxy-2'-methyl-3'-oxocyclopent-1'-en-1'-yl)-3-methylene-4-(3-oxobutyl)dihydrofuran-2(3H)-one (1), iso-seco-tanapartholide (2), 3-methoxy-tanapartho-lide (3), 3-acetyl-iso-seco-tanapartholide (4), cirsilineol (5), eriodictyol (6), nepetin (7), eupatorin (8), capillarisin (9), centaureidin (10), naringenin (11), homoeriodictyol (12), and eupatilin (13).In vitro anti-inflammatory assays showed that compound 1 inhibited the release of NO in RAW264.7 cells induced by LPS with IC₅₀ value of 31.15±2.30 μmol/L. Conclusion Compound 1 was a new compound, which was identified as 1R-tanaphillin, and compound 4 was isolated from Compositae for the first time. Compound 1 had certain anti-inflammatory activity.

R284.1

河南省高层次人才国际化培养项目（豫科[2021]72）；河南省重大科技专项（221100310400）