目的 研究担子菌棒柄杯伞Clitocybe clavipes的化学成分。方法 采用硅胶、Sephadex LH-20柱色谱和半制备液相等色谱学技术进行分离纯化，通过现代波谱学技术对化合物的结构进行表征和解析。同时采用MTT法测试化合物的体外细胞毒活性。结果 从棒柄杯伞95%乙醇提取物中分离鉴定了10个化合物，分别为 (1aR,11R,11aR,Z)-9-(aminomethyl)-7,10-dihydroxy- 5-methyl-3,6,11,11a-tetrahydro-2H-11,1a-(epoxymethano)benzo[4,5]cyclodeca[1,2-b]oxiren-13-one（1）、clavilactone C（2）、clavilactone D（3）、clavilactone F（4）、clavilactone G（5）、clavilactone J（6）、clavilactone K（7）、arnebinol A（8）、clavipyrrine A（9）、cyclo (Trp-Ala)（10）。化合物1～10对人胃癌HGC-27细胞具有不同程度的增殖抑制作用，其中化合物1、3、8、9的半数抑制浓度（median inhibition concentration，IC₅₀）分别为13.57、3.62、7.31、11.51 μmol/L。结论 化合物1为新化合物，命名为棒柄杯伞内酯Q。化合物10为首次从担子菌棒柄杯伞中分离得到；化合物1、3、8、9具有较为显著的细胞毒活性。

Objective To investigate the chemical constituents of Basidiomycete Clitocybe clavipes. Methods Chromatographic techniques including silica gel, Sephadex LH-20 column chromatography and semi-preparative liquid chromatography were employed to isolate and purify the compounds. Their structures were characterized and interpreted by modern wave spectroscopy techniques. The in vitro cytotoxic activity of compounds was determined by MTT assay. Results A total of ten compounds were isolated from the 95% EtOH extract of C. clavipes, which were identified as (1aR,11R,11aR,Z)-9-(aminomethyl)-7,10-dihydroxy- 5-methyl-3,6,11,11a-tetrahydro-2H-11,1a-(epoxymethano)benzo[4,5]cyclodeca[1,2-b]oxiren-13-one (1), clavilactone C (2), clavilactone D (3), clavilactone F (4), clavilactone G (5), clavilactone J (6), clavilactone K (7), arnebinol A (8), clavipyrrine A (9) and cyclo (Trp-Ala) (10). The results of cytotoxicity experiments showed that compounds 1—10 had different degrees of inhibitory effects on the proliferation of human gastric cancer cells HGC-27, and the median inhibition concentration (IC₅₀) values of compounds 1, 3, 8, and 9 were 13.57, 3.62, 7.31 and 11.51 μmol/L, respectively. Conclusion Compound 1 is a new compound named clavilactone Q, and compound 10 is isolated for the first time from Basidiomycete C. clavipes. Compounds 1, 3, 8, and 9 had significant cytotoxicity.

R284.1

国家自然科学基金项目（8216130391）