目的 基于关键氨基酸的含量差异，通过近红外技术实现淡水与海水珍珠的快速检测。方法 采用氨基酸分析仪考察108批次海水珍珠与淡水珍珠氨基酸种类与含量差异，依据氨基酸比较分析筛选关键氨基酸。针对关键氨基酸优化近红外定量模型，确立近红外光谱技术珍珠快速检测模型。结果 研究发现天冬氨酸（Asp）和甘氨酸（Gly）含量在淡水珍珠与海水珍珠有显著差异，且两者含量范围无交集。通过珍珠近红外光谱与Asp、Gly含量化学计量学拟合对珍珠样品进行检测，所得结果与传统的氨基酸分析技术测定结果一致。结论 基于化学计量学手段对珍珠的定量分析，结果准确可靠，结合近红外光谱技术对不同基原珍珠进行快速检测，为珍珠的质量控制提供方法依据。

Objective Based on Based on the difference of the content of key amino acids, the near infrared technology was used to detect the freshwater Margarita and seawater Margarita rapidly. Methods The amino acid analyzer was used to investigate the differences in the types and contents of amino acids between 108 batches of seawater Margarita and freshwater Margarita, and the key amino acids were screened according to the comparative analysis of amino acids. The near-infrared quantitative model was optimized for key amino acids, and the rapid detection model of Margarita was established by near infrared spectroscopy (NIRS). Results The results showed that the contents of aspartic acid (Asp) and glycine (Gly) were significantly different between freshwater Margarita and seawater Margarita, and there were no intersectional content range between Asp and Gly. The NIRS of Margarita was fitted with the stoichiometry of Asp and Gly content to detect Margarita samples, and the obtained results showed consistently with the determination results of traditional amino acid analysis technology. Conclusion The quantitative analysis of Margaritas based on stoichiometry showed that the results were accurate and reliable. The rapid detection of Margarita with different primordia was carried out combined with NIRS, providing a method basis for quality control of Margarita.

R286.2

中国（广西）-东盟药物创新中心建设及新药开发（桂科AA19254033）；2023年中央补助国家中医药管理局高水平中医药重点学科建设项目-海洋中药学（桂财社函〔2023〕97号）