目的 研究锦灯笼Physalis alkekengi var. franchetii宿萼95%乙醇提取物的化学成分与抗炎活性评价。方法 采用硅胶柱、Sephadex LH-20凝胶柱、HPLC制备等方法进行分离、纯化，通过波谱技术对化合物结构进行鉴定，采用脂多糖（lipopolysaccharide，LPS）诱导的RAW264.7小鼠单核巨噬细胞体外炎症模型进行抗炎活性评价。结果 分离得到14个化合物，分别鉴定为7β-ethoxy-25,27-dihydro-3,6-didehydroisophysalin M（1）、异酸浆苦素B（2）、4,7-didehydroneophysalin B（3）、酸浆苦素B（4）、25,27-dihydro-4,7-dedehydro-7-deoxyneophysalin A（5）、酸浆苦素E（6）、酸浆苦素L（7）、酸浆苦素O（8）、酸浆苦素D（9）、ergosta-4,24(28)-diene-3-one（10）、24-ethylcholesta-4,24(28)-dien-3-one（11）、sitosterone（12）、gramisterol（13）、柠黄醇（14）；在抗炎活性评价实验中，化合物2～6、8～9对NO生成具有抑制作用。结论 化合物1为新化合物，命名为酸浆苦素M1；化合物10～12、14均为首次从该属植物中分离得到；此外，化合物6对NO具有显著抑制作用，半数抑制浓度（median inhibition concentration，IC₅₀）值为 15.07 μmol/L，与阳性L-NMMA（IC₅₀＝36.11 μmol/L）相比具有较好的抗炎活性。

Objective To study the chemical composition and anti-inflammatory activity of 95% ethanol extract in the calyx of Jindenglong (Physalis alkekengi var. franchetii). Methods These compounds were separated and purified by silica gel column, Sephadex LH-20 gel column and HPLC preparation, and their structures were identified by spectroscopic techniques, the anti-inflammatory activities of RAW264.7 mouse mononuclear macrophages induced by LPS were evaluated in vitro. Results Fourteen compounds were isolated and their structures were identified as 7β-ethoxy-25,27-dihydro-3,6-didehydroisophysalin M (1), isophysalin B (2), 4,7-didehydroneophysalin B (3), physalin B (4), 25,27-dihydro-4,7-dedehydro-7-deoxyneophysalin A (5), physalin E (6), physalin L (7), physalin O (8), physalin D (9), ergosta-4,24(28)-diene-3-one (10), 24-ethylcholesta-4,24(28)-dien-3-one (11), sitosterone (12), gramisterol (13), citroflavin (14). In the experiment of anti-inflammatory activities evaluation, compounds 2—6, 8—9 had inhibitory effect on NO production. Conclusion Compounds 1 is a new compound named physalin M1. Compounds 10—12, 14 were isolated from this plant for the first time. In addition, compound 6 had a significant inhibitory effect on NO, and the IC₅₀ of compound 6 was 15.07 μmol/L, compared with positive L-NMMA (IC₅₀= 36.11 μmol/L), it had better anti-inflammatory activity.

R284.1

“十四五”国家重点研发计划课题（2022YFD1801103）