目的 研究连翘多胺氧化酶（polyamine oxidase，PAO）基因家族在盐和干旱胁迫下的响应特征。方法 通过分析连翘转录组数据鉴定出5个连翘PAO基因家族成员，结合蛋白质理化性质和基序分析、蛋白质结构分析、系统进化分析、结构域等方式进行相关性分析，并探究了盐和干旱胁迫下PAO基因的表达水平。结果 连翘PAO基因家族氨基酸数介于235～541 aa，相对分子质量在26 069～59 328，等电点在5.27～5.86；不稳定系数为35.68～44.14，总平均亲水性皆为负值，推测连翘PAO家族蛋白均为不稳定型、酸性、亲水性蛋白，α-螺旋与无规则卷曲为蛋白主要二级结构形式。结论 鉴定出的5个连翘PAO基因家族成员均具有特异性表达模式；在盐和干旱胁迫下5个连翘PAO基因家族成员的表达均受到诱导，可能参与连翘的盐胁迫及干旱胁迫响应调控，为深入探讨连翘PAO基因家族的功能奠定了基础。

Objective To study the response characteristics of Lianqiao (Forsythia suspensa) polyamine oxidase (FsPAO) gene family under salt and drought stress. Methods Five members of the FsPAO gene family were identified by analyzing the F. suspensa transcriptome data. The correlation analysis was carried out by means of protein physical and chemical properties and motif analysis, protein structure analysis, phylogenetic analysis, and structural domains. The gene expression levels were analyzed under salt and drought stress. Results The amino acid number of the FsPAO gene family ranged from 235 to 541 aa; The molecular weight from 26 069 to 59 328; The isoelectric point ranged from 5.27 to 5.86; The instability coefficients from 35.68 to 44.14, and the total average hydrophilicity was negative, so it was hypothesized that all the proteins of the FsPAO gene family were unstable, acidic, hydrophilic proteins, with α-Helix and irregular coil being the main secondary structural forms of PAO proteins. Conclusion The five identified members of the FsPAO gene family had specific expression patterns, and salt stress and drought stress induced the expression of the five members of the FsPAO gene family, which might be involved in the regulation of F. suspensa in response to salt stress and drought stress. Through this study lays a foundation for in-depth exploration of the function of the FsPAO gene family.

R286.12

国家自然科学基金项目（U1404829）；中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”（2060302）；河南省自然科学基金资助项目（202102110156）；河南省科技攻关（242102110325）；河南省中药材产业科技特派员服务团、河南省中药产业技术体系建设专项资金资助