目的 通过超高液相色谱串联三重四级杆飞行时间质谱法（UPLC-Triple-TOF-MS）对杜仲盐制前后成分进行鉴定，分析差异性成分。方法 利用UPLC-Triple-TOF/MS检测杜仲中的化学成分，液相采用Waters HSS-T3色谱柱（150 mm×2.1 mm，1.8 μm），以0.1%甲酸水溶液-0.1%甲酸乙腈溶液为流动相进行梯度洗脱，体积流量为0.3 mL/min，柱温为50 ℃；检测波长254 nm；进样量3 µL。质谱采用负离子扫描模式采集数据，根据一级准分子离子与二级碎片离子信息进行化合物的分析与鉴定。通过主成分分析（principal component analysis，PCA）和正交偏最小二乘法-判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA）对杜仲盐制前后进行差异成分分析。结果 共解析出杜仲中52种成分，结合OPLS-DA筛选出14个影响杜仲盐制前后质量的潜在差异性成分，分别为京尼平苷、绿原酸、杜仲醇、桃叶珊瑚苷、松脂醇二葡萄糖苷、京尼平苷酸、京尼平、eucomoside B、异绿原酸C、teuhircoside硫酸脂、橄榄素二葡萄糖苷、丁香脂素二葡萄糖苷、中脂素二葡萄糖苷和去氢二松柏醇葡萄糖苷。结论 建立了杜仲盐制前后化学成分定性分析方法，筛选出盐制前后杜仲质量变化的潜在差异性成分，为杜仲盐制的增效物质基础的研究提供了科学依据，对杜仲质量控制提供更加全面的支撑。

Objective To identify the components of Duzhong (Eucommiae Cortex, EC) before and after salt processing by ultra-high performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF-MS) and find out the differential components. Methods UPLC-Triple-TOF/MS was used to detect the chemical constituents in EC. The Waters HSS-T3 column (150 mm × 2.1 mm, 1.8 μm) was used as the liquid phase, and 0.1% formic acid solution-0.1% formic acid acetonitrile was used as the mobile phase for gradient elution. The volume flow rate was 0.3 mL/min, the column temperature was 50 ℃; and the detection wavelength was 254 nm. The injection volume was 3 μL. The data were collected by negative ion scanning mode, and the compounds were analyzed and identified according to the information of primary excimer ion and secondary fragment ion. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to analyze the quality difference of EC before and after salting. Results A total of 52 components in EC were analyzed, and 14 potential differential components affecting the quality of EC before and after salting were screened by OPLS-DA. They were geniposide, chlorogenic acid, eurycomanol, aucubin, pinoresinol diglucoside, geniposidic acid, genipin, eucomoside B, isochlorogenic acid C, teuhircoside sulfate, olivil 4′,4″-di-O-β-D-glucopyranoside, liriodendrin, medioresinol di-O-β-D-glucopyranoside and dehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside. Conclusion The qualitative analysis method of chemical components before and after salt processing of EC was established, and the potential differential components of the quality changes of EC before and after salt processing were screened out, which provided a scientific basis for the study of the synergistic material basis of EC salt processing, and provided a more comprehensive grasp of the quality control of EC.

浙江省自然科学基金项目（LTGN23H280001）；浙江省中医药科技计划中医药现代化专项项目（2020ZX006）；杭州市农业与社会发展科研重点项目（202204A06）；宁波市“科技创新2025”重大专项（2020Z089）