目的 对黑老虎Kadsura coccinea果实中的化学成分及抗炎活性进行研究。方法 通过硅胶、ODS、Sephadex LH-20、MCI等多种柱色谱技术进行分离、纯化，根据波谱数据和理化性质鉴定化合物的结构。构建脂多糖（lipopolysaccharide，LPS）诱导小鼠巨噬细胞RAW264.7释放NO的模型评价化合物的抗炎活性。结果 从黑老虎果实75%乙醇提取物中分离得到11个化合物，分别鉴定为 (aS,7R,8R,8'S,7'R)-7-苄酰氧基-7,7',8,8'-四氢-4,5-亚甲二氧基-3,3',4',5',7'-五甲氧基-8,8'-二甲基-二苯并[a,c]环辛烯（1）、3,4-二羟基-3',4'-二甲氧基-6,7'-环木脂素（2）、kadsuralignan H（3）、(＋)-(8S,8'R)-4,4'-二羟基-3,3',5'-三甲氧基木脂烷（4）、前戈米辛（5）、松脂醇（6）、aromadendrane-4β,10β-diol（7）、nephthediol（8）、oplodiol（9）、1β,11-dihydroxy-5-eudesmene（10）、pubinernoid A（11）。结论 化合物1为1个新的联苯环辛烯型木脂素类化合物，命名为血藤果素A；化合物2、4、7～11为首次从该属中分离得到。在50 μmol/L的浓度下，化合物4可以有效的抑制LPS诱导RAW264.7细胞NO的释放，抑制率为64.45%。

Objective To study the chemical constituents and anti-inflammatory activity in the fruits of Heilaohu [Kadsura coccinea (Lem.) A. C. Smith]. Methods The chemical constituents from fruits of K. coccinea were isolated and purified by silica gel, ODS, Sephadex LH-20, and MCI column chromatography. Their structures were determined by analysis of spectroscopic data and physicochemical properties, and by comparison with the reported data. All compounds were evaluated for their anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results A total of eleven compounds were isolated from 75% ethanol extract of the fruits of K. coccinea, and they were identified as (aS,7R,8R,8'S,7'R)-7-dibenzoyloxy-7,7',8,8'-tetrahydro-4,5-methylenedioxy-3,3',4',5',7'-pentamethoxy-8,8'-dimethyl-dibenzo[a,c]cycloocten (1), 3,4-dihydroxy-3',4'-dimethoxy-6,7'-cyclolignan (2), kadsuralignan H (3), (＋)-(8S,8'R)-4,4'-dihydroxy-3,3',5'-trimethoxylignan (4), pregomisin (5), pinoresinol (6), aromadendrane-4β,10β-diol (7), nephthediol (8), oplodiol (9), 1β,11-dihydroxy-5-eudesmene (10), pubinernoid A (11), respectively. Conclusion Compound 1 is a new dibenzocyclooctadiene lignan named kadcolignan A, compounds 2, 4, and 7—11 were isolated from this genus for the first time. At a concentration of 50 μmol/L, compounds 4 can significantly reduce the release of NO in LPS-stimulated RAW264.7 cells, with inhibition rates of 64.45%.

国家自然科学基金资助项目（81803847）；国家自然科学基金资助项目（82004063）；国家级大学生创新创业训练计划项目（202112214010）