目的 建立金银花药材超高效液相色谱（UPLC）指纹图谱，并对有效成分新绿原酸、绿原酸、隐绿原酸、咖啡酸、马钱苷、当药苷、断氧化马钱子苷、芦丁、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C的含量进行测定，为不同种质金银花药材的质量评价提供参考。方法 采用超高效液相色谱仪，Thermo Accucore^TM C₁₈色谱柱（100 mm×4.6 mm，2.6 μm），流动相为乙腈-0.1%磷酸水溶液，体积流量为0.3 mL/min，梯度洗脱，分段检测波长324 nm（0～13.5 min）、238 nm（13.5～18 min）、354 nm（18～27 min）、324 nm（27～40 min），柱温30 ℃，进样量2 μL；通过对照品比对，对共有峰进行鉴定，采用《中药色谱指纹图谱相似度评价系统（2012版）》软件构建指纹图谱，进行相似度评价；通过主成分分析（principal component analysis，PCA）、正交偏最小二乘法判别分析（orthogonal partial least-squares discrimination analysis，OPLS-DA）比较不同种质金银花药材的质量差异；并对不同种质金银花药材12种成分的含量进行测定。结果 建立10种不同种质金银花指纹图谱，标定共有峰11个，并指认了9个化学成分，分别为新绿原酸（1号峰）、绿原酸（2号峰）、隐绿原酸（3号峰）、马钱苷（4号峰）、断氧化马钱子苷（5号峰）、木犀草苷（7号峰）、异绿原酸B（8号峰）、异绿原酸A（9号峰）、异绿原酸C（11号峰）；相似度评价结果显示，10种金银花指纹图谱相似度在0.723～0.996，除S23的相似度为0.723以外，其余金银花相似度均大于0.91；运用PCA和OPLS-DA将6批金银花明显区分，OPLS-DA筛选出4个差异性标志物，根据VIP值排序，分别为断氧化马钱子苷＞新绿原酸＞马钱苷＞隐绿原酸；含量测定结果显示，不同种质金银花的各发育期成分含量变化不同。结论 通过金银花UPLC指纹图谱与多成分化学模式识别研究相结合，可有效评价不同种质金银花药材的质量差异性，为不同种质金银花药材的质量控制提供参考。

Objective To establish ultra-high performance liquid chromatography (UPLC) fingerprint of Jinyinhua (Lonicerae Japonicae Flos), and to determine the contents of effective components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, loganin, sweroside, secoxyloganin, rutin, luteoloside, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, in order to provide reference for quality evaluation of different germplasm of Lonicerae Japonicae Flos. Methods The analysis was performed on an UPLC (Agilent 1290) with Thermo Accucore^TM C₁₈ column (100 mm×4.6 mm, 2.6 μm) with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution for gradient elution, with flow rate of 0.3 mL/min, detection wavelength of 324 nm (0—13.5 min), 238nm (13.5—18 min), 354 nm (18—27 min), 324 nm (27—40 min), column temperature of 30 ℃, and injection volume of 2 μL. The common peaks were identified by comparison of reference materials. The software "TCM Chromatographic Fingerprint Similarity Evaluation System (2012 Edition)" was used to construct the fingerprint and evaluate the similarity.Through principal component analysis (principal component analysis, PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to compare the quality of Lonicerae Japonicae Flos with different germplasm. The contents of 12 components in different germplasm of Lonicerae Japonicae Flos were determined. Results The fingerprints of 10 different germplasm Lonicerae Japonicae Flos were established, 11 peaks were identified, and nine chemical components were identified. They were neochlorogenic acid (peak 1), chlorogenic acid (peak 2), cryptochlorogenic acid (peak 3), loganin (peak 4), secoxyloganin (peak 5), luteoloside (peak 7), isochlorogenic acid B (peak 8), isochlorogenic acid A (peak 9), and isochlorogenic acid C (peak 11). The similarity evaluation results showed that the similarity of 10 kinds of honeysuckle fingerprints ranged from 0.723 to 0.996, except for the similarity of S23 which was 0.723, the similarity of other kinds of honeysuckle was greater than 0.91. Six batches of Lonicerae Japonicae Flos were distinguished by PCA and OPLS-DA. Four different markers were selected by OPLS-DA. According to the VIP value, they were secoxyloganin > neochlorogenic acid > loganin > cryptochlorogenic acid. The results of content determination showed that the content of Lonicerae Japonicae Flos varied in different germplasm at different development stages. Conclusion The combination of UPLC fingerprint and multi-component chemical pattern recognition of Lonicerae Japonicae Flos can effectively evaluate the quality difference of different germplasm of Lonicerae Japonicae Flos, and provide reference for quality control of different germplasm of Lonicerae Japonicae Flos.

山东省科技型中小企业创新能力提升工程（2023TSGC0444）；山东省中医药高层次人才培育项目专项经费资助（2023143）；全国中药特色技术传承人才培训项目（202396）；中药资源保障能力提升工程（202319）；临沂市重点研发计划项目（2022026）；国家重点研发计划（2017YFC1701500）；中央本级重大增减支项目（2060302）；济南市农业应用技术创新计划项目（CX202112）