目的 建立经典名方茵陈蒿汤（Yinchenhao Decoction，YD）基准样品的HPLC指纹图谱并对其指标性成分（绿原酸、栀子苷、对羟基苯乙酮、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）进行含量测定，研究YD基准样品的量值传递规律。方法 制备15批YD基准样品，建立HPLC指纹图谱，明确其指纹图谱与对照指纹图谱（R）的相似度，共有峰的峰归属，出膏率范围，对指标性成分的含量及转移率进行分析。结果 15批YD基准样品指纹图谱与R的相似度均大于0.900；共归属33个共有峰，经对照品指认8个共有峰信息，其中峰1、6、10、11、13（对羟基苯乙酮）、15、19、20、22来源于茵陈，峰3、7、8、12（栀子苷）、14、21、23、24、27、28来源于栀子，峰2、16、17、18、25、26、29~33（芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）来源于大黄，峰4、5、9（绿原酸）为茵陈和栀子所共有；15批基准样品的出膏率为23.170%~29.952%；指标性成分绿原酸、栀子苷、对羟基苯乙酮、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚质量分数分别为0.107%~0.282%、3.153%~5.757%、0.010%~0.035%、0.008%~0.039%、0.007%~0.147%、0.003%~0.008%、0.003%~0.012%、0.001%~0.003%，转移率分别为32.290%~61.345%、40.365%~81.823%、66.473%~93.596%、7.398%~35.966%、4.971%~91.091%、1.841%~16.085%、0.599%~6.922%、0.173%~1.965%；15批基准样品中5种游离总蒽醌的质量分数为0.033%~0.185%，转移率为2.081%~40.274%。结论 采用指纹图谱及多指标性成分含量测定相结合的模式，对经典名方YD基准样品的量值传递过程进行分析，可为YD基准样品的质量控制及复方制剂的开发提供科学依据。

Objective To establish the HPLC fingerprint of benchmark samples of a classical famous prescription, Yinchenhao Decoction (YD, 茵陈蒿汤), determine the content of its multi-index components including chlorogenic acid, geniposide, 4'-hydroxyacetophenone, aloeemodin, rhein, emodin, chrysophanol and physcion, and explore the rules for the quality value transfer of benchmark samples in YD. Methods By preparing 15 batches of benchmark samples of YD, the methodology of the fingerprints of benchmark samples was established. Furthermore, the similarity range between these fingerprints and the reference fingerprint (R) and the identification of attribution of common peaks were explored. The paste-forming rate range was determined, the contents and the transfer rate range of index components were analyzed. Results The 15 batches of the benchmark samples fingerprints had good similarities with R, which were all higher than 0.900. 33 Commom peaks were assigned, and eight commom peaks were assigned by the reference substance, among which, peaks 1, 6, 10, 11, 13 (4'-hydroxyacetophenone), 15, 19, 20, 22 originated from Yinchen (Artemisiae Scopariae Herba, ASH); Peaks 3, 7, 8, 12 (geniposide), 14, 21, 23, 24, 27, 28 came from Zhizi (Gardeniae Fructus, GF); Peaks 2, 16, 17, 18, 25, 26, 29-33 (aloeemodin, rhein, emodin, chrysophanol and physcion) came from Dahuang (Rhei Radix et Rhizoma, RRR), and peaks 4, 5, 9 (chlorogenic acid) were the common peaks of ASH and GF. The paste-forming rate of the 15 batches of benchmark samples ranged from 23.170% to 29.952%. The mass fraction ranges of chlorogenic acid, geniposide, 4'-hydroxyacetophenone, aloeemodin, rhein, emodin, chrysophanol and physcion were 0.107%-0.282%, 3.153%-5.757%, 0.010%-0.035%, 0.008%-0.039%, 0.007%-0.147%, 0.003%-0.008%, 0.003%-0.012%, and 0.001%-0.003%, respectively. The transfer rates ranged from 32.290% to 61.345%, 40.365% to 81.823%, 66.473% to 93.596%, 7.398% to 35.966%, 4.971% to 91.091%, 1.841% to 16.085%, 0.599% to 6.922%, and 0.173% to 1.965%, respectively; The mass fraction range of free total anthraquinone in the 15 batches of YD benchmark samples was 0.033%-0.185%, and the transfer rate ranged from 2.081% to 40.274%. Conclusion By using the combination of fingerprint and the content determination of the multi-index components, the quality value transfer of benchmark samples in YD can be analyzed, which provides a scientific basis for the quality control of the benchmark samples and the development of compound preparations.

R283.6

四川省科技厅-泸州市政府-泸州医学院三方联合专项（14ZC0045）；四川省科技支撑计划项目（2010SZ0049）