目的 制备载冬凌草甲素和黄芪多糖-丝素蛋白-甲基纤维素（oridonin & Astragalus polysaccharides-silk fibroin-methyl cellulose，Ori&APS-SF-MC）复合伤口敷料，并评价其对皮肤伤口愈合的效果。方法 采用冷冻干燥法成功制备了Ori&APS-SF-MC复合伤口敷料，通过红外光谱及扫描电子显微镜考察复合伤口敷料的相容性，并对其外观形态、包封率、载药量和体外释放行为进行表征，以金黄色葡萄球菌与大肠杆菌为菌种，利用抑菌圈法和涂布平板法检测复合伤口敷料的抑菌性能。最后通过小鼠背部全层皮肤厚度伤口模型观察伤口愈合情况与伤口组织学形态以评价Ori&APS-SF-MC复合伤口敷料对皮肤伤口愈合的效果。结果 冬凌草甲素、黄芪多糖及丝素蛋白具有良好的相容性，可成功制备Ori&APS-SF-MC复合伤口敷料；其冬凌草甲素的包封率为（99.70±0.04）%，载药量为（3.66±0.05）%；黄芪多糖的包封率为（92.47±0.14）%，载药量为（83.57±0.12）%；体外释药性能优异。随着冬凌草甲素质量浓度的增加，对金黄色葡萄球菌抗菌能力增强，但对大肠杆菌无抑制作用。相较于其他组，Ori&APS-SF-MC组处理后皮肤受损处的红肿、出血、感染、渗出液情况明显减少，伤口愈合率也相对升高。病理结构显示，Ori&APS-SF-MC组小鼠皮肤损伤表面基本愈合，表皮增厚，炎性细胞浸润较少、成纤维细胞和新生的毛细血管较多、胶原纤维排列较规则有序。结论 Ori&APS-SF-MC复合伤口敷料可促进皮肤伤口愈合。

Objective To prepare oridonin & Astragalus polysaccharides-silk fibroin-methyl cellulose (Ori&APS-SF-MC) composite wound dressing and evaluate its effect on skin wound healing. Methods Ori&APS-SF-MC composite wound dressing was prepared successfully using the freeze-drying method. The compatibility of the composite wound dressing was investigated using infrared spectroscopy and scanning electron microscopy. The appearance, encapsulation efficiency, drug loading, and in vitro release behavior were characterized. Staphylococcus aureus and Escherichia coli were used to test the antibacterial properties of the composite wound dressing by inhibition zone method and coating plate method. Finally, the effect of Ori&APS-SF-MC composite wound dressing on skin wound healing was evaluated by observing the wound healing and histological morphology of a full-thickness skin wound model on the back of mice. Results Oridonin, Astragalus polysaccharides and silk fibroin exhibited good compatibility, and Ori&APS-SF-MC composite wound dressing was successfully prepared. The encapsulation efficiency of oridonin was (99.70 ±0.04)%, and the drug loading was (3.66 ±0.05)%. The encapsulation efficiency of Astragalus polysaccharides was (92.47 ±0.14)%, and the drug loading was (83.57 ±0.12)%. The antibacterial ability against S. aureus was enhanced with the increase of oridonin concentration, but no inhibitory effect was observed on E. coli. Compared with other groups, the Ori&APS-SF-MC group showed reduced swelling, bleeding, infection and exudate in damaged skin, as well as an increased wound healing rate. The pathological structure analysis revealed that the surface of skin injury in the Ori&APS-SF-MC group was mostly healed, with thickened epidermis, less infiltration of inflammatory cells, increased fibroblasts and new capillaries, and regular arrangement of collagen fibers. Conclusion Ori&APS-SF-MC composite wound dressing can promote skin wound healing.

R283.6

国家重点研发计划（2018YFC1708005）；国家留学基金委青年骨干教师项目（201800850005）；西南民族大学科研资助项目（2023KYZZ06N）