目的 建立茯神HPLC指纹图谱及10个三萜类成分（16α-羟基松苓新酸、茯苓酸B、去氢土莫酸、茯苓酸A、多孔菌酸C、3-表去氢土莫酸、3-O-乙酰基-16α-羟基松苓新酸、去氢茯苓酸、松苓新酸、去氢齿孔酸）的含量测定方法。方法 通过HPLC建立茯神指纹图谱，对37批茯神10个成分的含量进行测定。并结合相似度评价、聚类分析（cluster analysis，CA）、主成分分析（principal component analysis，PCA）及正交偏最小二乘判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）对茯神质量进行综合评价。结果 37批茯神HPLC特征图谱共匹配10个共有峰成分，除S23、S37外，其余样品间相似度为0.791～1.000；CA将37批样品分为3类，PCA提取了2个主成分，OPLS-DA确定去氢茯苓酸、16α-羟基松苓新酸、多孔菌酸C、3-表去氢土莫酸4个质量差异标志物。结论 建立的指纹图谱及含量测定方法操作简便、专属性强、重复性好，可为茯神的质量控制提供参考。

Objective To establish a HPLC characteristic chromatogram of Fushen (Poria cum Pini Radix) and a method for determining the content of 10 triterpenoid components (16α-hydroxydehydrotrametenolic acid, poricoic acid B, dehydrotumulosic acid, poricoic acid A, polyporenic acid C, 3-epidehydrotumulosic acid, 3-O-acetyl-16α-hydroxydehydrotrametenolic acid, dehydropachymic acid, 3β-hydroxy-5α-lanosta-7,9(11),24-trien-21-oic acid, dehydroeburicoic acid). Methods The content of 10 components in 37 batches of Poria cum Pini Radix was determined by establishing HPLC characteristic chromatogram. And similarity evaluation, cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to comprehensively evaluate the quality of Poria cum Pini Radix. Results The HPLC characteristic chromatogram were matched with 10 common peaks in 37 batches of Poria cum Pini Radix. Except for S23 and S37, the similarity between the other samples was 0.791−1.000; CA divided 37 batches of samples into three categories, PCA extracted two principal components, and OPLS-DA determined four quality difference markers named dehydropachymic acid, 16α-hydroxydehydrotrametenolic acid, polyporenic acid C, 3-epidehydrotumulosic acid. Conclusion The established characteristic chromatogram and content determination method are easy to operate, have strong specificity and good repeatability, which can provide reference for the quality control of Poria cum Pini Radix.

安徽省药品监督管理局监管科学研究重点项目：茯神产业化关键技术及质量标准研究（AHYJ-KJ-202310）