目的 通过实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）筛选当归Angelica sinensis不同材料中稳定表达的内参基因。方法 以不同生长期、春化温度、组织部位以及抽薹/未抽薹植株为材料，对转录组数据库获得的13个候选内参基因和1个已报道的肌动蛋白基因的表达水平进行qRT-PCR检测，利用geNorm、NormFinder、BestKeeper、∆C_t和RefFinder等软件分析表达水平的稳定性。结果 不同材料中14个候选内参基因的稳定性存在显著差异，ACT7、UBC32、UBC26和UBC7分别为不同发育阶段、春化温度、组织部位和抽薹/未抽薹植株中最稳定的内参基因；EEF1G为所有样品中最稳定的内参基因，其次为RPL3和UBC26。结论 为进一步检测当归产量和品质形成过程中的基因表达提供重要参考。

Objective Selecting the stable expression of reference genes in different materials of Angelica sinensis by using the method of quantitative real-time PCR (qRT-PCR). Methods The different growth stages, vernalization temperatures, tissue parts and bolted /unbolted plants were used as materials, the expression levels of 13 candidate reference genes obtained from the transcriptome database and one Actin gene reported in previous literatures were quantified by the qRT-PCR, and the stability of expression levels was analyzed by the software of geNorm, NormFinder, BestKeeper, ∆C_t and RefFinder. Results There were significant differences in the stability of the 14 candidate reference genes in the different samples. The genes ACT7, UBC32, UBC26 and UBC7 were the most stable reference gene in different growth stages, vernalization temperatures, tissue parts, and bolted/unbolted plants, respectively. The EEF1G gene was the most stable reference gene in all materials, followed by the RPL3 and UBC26 genes. Conclusion These findings will provide a critical reference for further detecting the gene expression during the formation of yield and quality of A. sinensis.

国家重点研发计划（2022YFC3501500）；财政部和农业农村部：国家现代农业产业技术体系（CARS-21）；国家自然科学基金项目（32160083）；甘肃省科技厅重点研发计划（22YF7NA111）