[关键词]
[摘要]
目的 研究红簕钩Rubus pirifolicus的化学成分。方法 采用大孔树脂、硅胶、ODS、Sephadex LH-20以及半制备HPLC等方法进行分离纯化，根据波谱数据并结合文献对化合物结构进行鉴定。结果 从红簕钩醋酸乙酯部位中分离得到16个化合物，分别鉴定为梨叶悬钩子酸A（1）、acanthochlamic acid（2）、2α,3β-dihydroxyolean-13(18)-en-28-oic acid（3）、3-表-2-氧代-坡模醇酸（4）、2α,3α,19α,22α-tetrahydroxyurs-12-en-28-oic acid（5）、23-O-galloylarjungenin（6）、2α,3α,19α,24-四羟基齐墩果-12-烯-28-羧酸（7）、bellericagenin B（8）、roburgenic acid（9）、2β,3β,19α-三羟基齐墩果-12-烯-23,28-二羧酸（10）、2α,3β,23-trihydroxylup-20(29)-en-28-oic acid 28-O-β-D-glucopyranoside（11）、熊果酸（12）、山楂酸（13）、科罗索酸（14）、坡模酸（15）、齐墩果酸（16）。结论 化合物1为新化合物，命名为梨叶悬钩子酸A，化合物2～3、5～6、8～10为首次从悬钩子属中分离得到，所有化合物均为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from Rubus pirifolicus. Methods The extract from R. pirifolicus was isolated and purified by macroporous resin, silica gel column, ODS, Sephadex LH-20 and semi-preparative HPLC. Their structures were identified by spectral analysis combined with literature. Results Sixteen compounds were isolated from the ethyl acetate extracts of R. pirifolicus and identified as pirifoliusic acid A (1), acanthochlamic acid (2), 2α,3β-dihydroxyolean- 13(18)-en-28-oic acid (3), 3-epi-2-oxopomolic acid (4), 2α,3α,19α,22α-tetrahydroxyurs-12-en-28-oic acid (5), 23-O-galloylarjungenin (6), 2α,3α,19α,24-tetrahydroxyolean-12-en-28-oic acid (7), bellericagenin B (8), roburgenic acid (9), 2β,3β,19α-trihydroxyolean- 12-ene-23,28-dioic acid (10), 2α,3β,23-trihydroxylup-20(29)-en-28-oic acid 28-O-β-D-glucopyranoside (11), ursolic acid (12), maslinic acid (13), corosolic acid (14), pomolic acid (15), oleanolic acid (16). Conclusion Compound 1 is a new compound and named pirifoliusic acid A; Compounds 2—3, 5—6, 8—10 were isolated from the Rubus for the first time, and all the compounds were isolated from this plant for the first time.
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[基金项目]
国家重点研究计划项目（2019YFC1712304）；广西科技基地和人才专项（桂科AD20238058）；广西中医药管理局科研课题（GXZYZ20210068）；广西壮瑶药重点实验室项目（GXZYKF2022-16）；广西中医药大学科研项目（2020ZD002）；广西中医药大学研究生教育创新计划项目（YCXJ2021020）；国家级大学生创新创业训练计划项目（202110600013）
