目的 建立葛根Pueraria lobate药材HPLC指纹图谱，并测定不同产地葛根中6种成分（3'-羟基葛根素、葛根素、3'-甲氧基葛根素、葛根素芹菜糖苷、大豆苷、大豆苷元）的含量，为葛根优质药材产地与质量控制提供参考。方法 采用HPLC法，葛根药材30%乙醇提取液的分析采用Hypersil BDS C₁₈色谱柱（250 mm×4.6 mm，5 μm）；0.1%甲酸水-乙腈为流动相，梯度洗脱；体积流量1.0 mL/min；检测波长250 nm；柱温25℃。建立32批葛根药材指纹图谱，并测定不同产地葛根药材6种成分的含量。结果 建立了葛根药材HPLC指纹图谱，共提取出10个共有峰，指认6个共有峰；建立了含量测定方法，并对不同产地来源的32批葛根药材中6种成分含量测定，不同产地葛根质量对比综合评分结果显示河南最高，其次为陕西。结论 建立了葛根HPLC指纹图谱方法，通过对比，各样品与对照图谱相似度均大于0.9，为葛根道地药材质量标准提供整体质量控制依据。结合产地调研信息，可将葛根优质原料药材产地优选为陕西、河南秦岭山脉。

Objective To establish the HPLC fingerprints of Gegen [Puerariae Lobatae Radix, PLR] and to determine the six components (3'-hydroxypuerarin, puerarin, 3'-methoxy puerarin, puerarin apigenin, Daidzin and Daidzein) in PLR from different regions, providing reference for the high-quality origin and quality control of PLR. Methods Hypersil BDS C₁₈ column (250 mm×4.6 mm, 5μm) was used for the analysis of 30% ethanol extract of PLR by HPLC. The mobile phase was 0.1% formic acid-acetonitrile, with the detection wavelength of 250 nm, column temperature of 25℃ and volume flow rate of 1.0 mL/min for gradient elution. The fingerprint of 32 batches of PLR was established, and the content of six components of PLR were determined. Results HPLC fingerprints of PLR were established, nine common peaks were extracted and six common peaks were identified. The content determination method was established, and the content of six components in 32 batches of PLR from different origin was determined. The comprehensive score of quality comparison of PLR from different origin showed that Henan was the highest, followed by Shaanxi. Conclusion This study established a HPLC fingerprint method for PLR. Through comparison, the similarity between each sample and the control spectrum was greater than 0.9, providing an overall quality control basis for the quality standard of genuine PLR. Based on the research information of the production area, the high-quality raw material of PLR can be selected as the Qinling Mountains in Shaanxi and Henan.

R286.2

国家中药标准化项目（ZYBZH-C-GD-07）；2018年工业转型升级资金（部门预算）项目（0714-EMTC-02-00576）