目的 建立一测多评（quantitative analysis of multicomponents by single-marker，QAMS）法同时检测盐沙苑子饮片中杨梅素、扁蓄苷、杨梅素-3-O-β-D-葡萄糖苷、毛蕊异黄酮葡萄糖苷、西伯利亚落叶松黄酮、沙苑子苷B、沙苑子苷A、毛蕊异黄酮、芒柄花素、鼠李柠檬素、豆甾醇、胡萝卜苷、β-谷甾醇含量的方法，并采用主成分分析法（principal component analysis，PCA）、正交偏最小二乘法-判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA）法及熵权逼近理想解排序（entropy weight-technique for order preference by similarity to ideal solution，EW-TOPSIS）法对其质量进行评价。方法 采用Lichrospher C₁₈色谱柱；乙腈-0.2%磷酸水溶液为流动相梯度洗脱。以沙苑子苷A为内参物，建立内参物与其他12个待测成分的相对校正因子（relative correction factor，RCF），进行RCF耐用性考察及色谱峰定位，并与外标法实测结果进行对比。运用OPLS-DA及EW-TOPSIS法建立盐沙苑子饮片综合质量优劣评价方法。结果 方法学验证均符合《中国药典》2020年版要求；QAMS和外标法含量测定结果无显著性差异（P>0.05）；沙苑子苷A、β-谷甾醇、沙苑子苷B、杨梅素、胡萝卜苷和毛蕊异黄酮是影响盐沙苑子饮片产品质量的主要潜在标志物；加权TOPSIS法结果显示陕西地区所得盐沙苑子饮片质量最优，其次为山西、河北和山东产盐沙苑子饮片，辽宁和内蒙古产盐沙苑子饮片位于排名后4位。结论 所建立的方法操作便捷、结果准确；OPLS-DA联合EW-TOPSIS法全面客观，可用于盐沙苑子饮片综合品质的评价。

Objective To establish an quantitative analysis of multicomponents by single-marker (QAMS) method for simultaneous determination of myricetin, avicularin, myricetin 3-O-β-D-glucopyranoside, calycosin 7-O-β-D-glucopyranoside, laricitrin, complanatoside B, complanatuside A, calycosin, formononetin, rhamnocitrin, stigmasterol, daucosterol and β-sitosterol in salt-Astragali Complanati Semen (sACS), and to evaluate its quality by principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and entropy weight -technique for order preference by similarity to ideal solution method (EW-TOPSIS). Methods QAMS method was developed on a Lichrospher C₁₈ column with acetonitrile-0.2% phospic acid solution as mobile phase for a gradient elution. Using complanatuside A as a reference, the relative correction factor (RCF) of the internal reference and the other twelve components to be tested was established, and the RCF durability and chromatographic peak positioning were investigated. At the same time, the results were compared with those measured by the external standard method. OPLS-DA and EW-TOPSIS method were used to analyze the comprehensive quality evaluation of sACS. Results The methodological verification met the requirements of Chinese Pharmacopoeia 2020 Editio. There was no significant difference in the determination results of QAMS method and external standard method (P > 0.05). Complanatuside A, β-sitosterol, complanatoside B, myricetin, daucosterol and calycosin were the main potential markers affecting the quality of sACS. The results of EW-TOPSIS method showed that the quality of sACS in Shaanxi was the best, followed by Shanxi, Hebei and Shandong, Liaoning and Inner Mongolia were in the last four places. Conclusion The established method is convenient and accurate, and OPLS-DA combined with EW-TOPSIS method was comprehensive and objective, and could be used for the comprehensive evaluation of the quality of sACS.

R283.6

天津市高等职业技术教育研究会课题项目（XVII4016）