目的 采用HPLC指纹图谱与抗氧化活性相关联，建立谱-效关系，旨在分析筛选佛手Citrus medica var. sarcodactylis抗氧化活性的有效成分。方法 采集24批次佛手药材的指纹图谱，在对照图谱相似度评价均大于0.880的基础上，结合聚类热图及主成分分析法（PCA）对指纹图谱数据进行研究；采用DPPH自由基和ABTS自由基清除法评价佛手24批次样品的抗氧化活性；通过灰色关联度法对24批次佛手共有峰与其抗氧化能力指标进行关联性分析，综合评价筛选有效成分。结果 从24批次佛手样品指纹图谱中确定了17个共有峰，通过对照品比对法指认了其中10个化合物。聚类热图分析与抗氧化结果显示，24批次佛手大致被分为3类；且均有较好的抗氧化活性，并呈浓度依赖性，其中S16的抗氧化活性最强。综合灰色关联度分析和综合评分结果，F16（香叶木素）、F11、F12（5,7-二甲氧基香豆素）和F8（橙皮苷）化合物可能是反映佛手药效的重要特征峰，与主成分中展现的重要峰信息基本一致。结论 通过佛手24批次HPLC指纹图谱与抗氧化活性之间的相关性分析，可为挖掘佛手的有效成分以及药材质量控制提供参考。

Objective The HPLC fingerprints were correlated with the antioxidant activity to establish a spectrum-effect relationship, aiming to analyze and screen the active ingredients with the antioxidant activities of Foshou (Citri Sarcodactylis Fructus) (Citrus medica var. sarcodactylis).Methods The fingerprint profiles of 24 batches of Citri Sarcodactylis Fructus were collected, and the fingerprint data were studied by combining the clustering heat map and principal component analysis (PCA) method on the basis of the similarity evaluation of the control profiles, which were all greater than 0.880. The antioxidant activities of 24 batches of Citri Sarcodactylis Fructus samples were evaluated by the DPPH and ABTS radical scavenging methods; The correlation analysis of the 24 batches of Citri Sarcodactylis Fructus common peaks with their antioxidant capacity indexes was carried out by the gray correlation method to comprehensively evaluate the screened active ingredients. Results Seventeen common peaks were identified from the fingerprint profiles of 24 batches of Citri Sarcodactylis Fructus samples, and 10 of which were identified by the control comparison method. The clustering heat map analysis and antioxidant test results showed that the 24 batches of Citri Sarcodactylis Fructus were roughly classified into three categories, and all of them had good antioxidant activity and showed concentration dependence, among which S16 had the strongest antioxidant activity. Combining the results of gray correlation analysis and comprehensive scoring, the compounds F16 (diosmetin), F11, F12 (5,7-dimethoxycoumarin), and F8 (aurantiamarin) maybe important characteristic peaks reflecting the efficacy of Citri Sarcodactylis Fructus, which were basically consistent with the information of important peaks exhibited in the main components. Conclusion By analyzing the correlation between the antioxidant activity of 24 batches of Citri Sarcodactylis Fructus and the HPLC fingerprint profiles, this study can serve as a guide for identifying active ingredients and quality control of Citri Sarcodactylis Fructus.

R286.2

重庆市基本科研业务费项目（cstc2021jxjl-jbky10004）；农业部国家中药材产业技术体系（CARS-21）；重庆市技术创新与应用发展重点专项项目（cstc2021jscx-dxwtBX0011，CSTB2022TIAD-KPX0094）