目的 以完善红芪Hedysari Radix质量评价方法与内容为目的，建立红芪HPLC指纹图谱并测定4种有效成分的含量，并在建立多成分含量测定方法基础上，为其质量标志物的可测性提供依据。方法 运用HPLC法建立36批红芪的指纹图谱，通过对照品比对，对共有峰进行指认，确定并建立4种有效成分含量的测定方法，在指纹图谱相似度评价基础之上，运用聚类分析、主成分分析、判别分析对36批次红芪差异性成分进行分析。结果 36批红芪指纹图谱共有16个峰，指认了4个峰，分别是毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素；30批红芪相似度大于0.900，6批红芪的相似度在0.802～0.900；聚类分析将36批红芪分为4类；主成分因子分析提取了4个主成分，5批红芪主成分综合得分较低，与其他批次有较大差异，主成分分析依据生长类型将红芪分为2类、依据商品等级分为4类，差异性成分分别为峰7、芒柄花苷、峰10、毛蕊异黄酮苷、芒柄花素；判别分析结果与主成分分析一致；毛蕊异黄酮苷、芒柄花苷以及4种有效成分的总量与主成分综合得分呈显著负相关，指认的4种有效成分在可测性方面具备成为红芪质量标志物的科学性。结论 建立的HPLC指纹图谱和多成分含量测定结合化学模式识别法的评价模式，能够有效分析红芪质量的差异性，为现有红芪质量控制与评价提供研究基础和参考依据。

Objective In order to improve the quality evaluation method and content of Hongqi (Hedysari Radix), this study established HPLC fingerprint of Hongqi (Hedysari Radix) and determined the contents of four active components. Based on the establishment of multi-component content determination method, it provided the basis for the measurability of its quality markers. Methods HPLC method was used to establish the fingerprint of 36 batches of Hedysari Radix. The common peaks were identified by comparison with reference products, and four methods for the determination of active components were determined and established. On the basis of fingerprint similarity evaluation, cluster analysis, principal component analysis and discriminant analysis were used to analyze the differential components of 36 batches of Hedysari Radix.Results There were 16 peaks in 36 batches of Hedysari Radix fingerprints, and four peaks were identified, which were calycosin-7-glycoside, ononin, calycosin and formononetin; The similarity of 30 batches was greater than 0.900, and the similarity of six batches was 0.802—0.900. The 36 batches of Hedysari Radix were divided into four groups by cluster analysis; Four principal components were extracted by principal component factor analysis, and the comprehensive scores of principal components of the five batches of Hedysari Radix were low, which were significantly different from the other batches. Hedysari Radix was divided into two categories according to the growth type, and four categories according to the commodity grade. The differential components were peak 7, ononin, peak 10, calycosin-7-glucoside and formononetin; The results of discriminant analysis were consistent with those of principal component analysis; There was a significant negative correlation between the total amount of calycosin-7-glucoside, ononin and four active components and the comprehensive score of principal components. The four active ingredients identified became the scientific quality markers of Hedysari Radix in the aspect of measurability. Conclusion The established the HPLC fingerprint and multiple component content quantitative method combined with the combination of chemical pattern recognition method can effectively analyze the quality of Hedysari Radix, with view to providing research foundation and reference basis for the quality control and evaluation of Hedysari Radix.

R286.2

兰州市城关区科技计划项目（2020-2-2-2）；甘肃省科技计划自然科学基金项目（21JR11RA136）；甘肃省教育厅双一流重大科研项目（GSSYLXM-05）；甘肃省中药制药工艺工程研究中心开放课题（ZYGY202004）；中医药公共卫生服务补助专项子课题（2305191901）