目的 TCP基因家族在植物叶片发育、侧枝形成、花器官形成、植物激素合成与信号转导等生理过程中发挥着关键的作用。对工业大麻Cannabis sativa TCP基因进行全基因组鉴定及表达分析为工业大麻TCP基因家族的功能分析提供科学参考。方法 基于已发布的工业大麻基因组，利用生物信息学方法对工业大麻TCP基因家族成员进行鉴定并对其理化性质、基因结构、以及表达模式进行分析。结果 共鉴定出17个工业大麻TCP基因（CsTCP1～CsTCP17）分为2类3个亚科（PCF、CIN和CYC/TB1），编码的氨基酸长度在163～585 aa，蛋白质相对分子质量为18 310～64 070，蛋白等电点介于4.99～9.36，亚细胞定位大部分为细胞核。共线性进化分析发现，工业大麻与拟南芥、番茄、水稻分别存在9、20和6对同源基因。顺式元件预测结果表示光响应元件和脱落酸元件在TCP基因启动子区域分布广泛。基因表达分析结果表明，工业大麻TCP基因的表达存在组织特异性，大部分在叶和花中高表达。结论 为深入研究工业大麻TCP基因家族的功能奠定了基础，进一步阐明了TCP基因可能参与工业大麻生长发育的各个阶段，并对工业大麻的药用资源发展提供理论基础。

Objective The TCP gene family plays a crucial role in plant development and physiological processes. This study provides a scientific reference for the functional analysis of the TCP gene family in Gongyedama (Cannabis sativa) through . Methods Based on the published C. sativa genome, bioinformatics methods were used to identify the TCP gene family members of C. sativa and analyze their physicochemical properties, gene structure, and expression patterns. Results A total of 17 C. sativa TCP genes (CsTCP1—CsTCP17) were identified, divided into two categories and three subfamilies (PCF, CIN, and CYC/TB1), encoding amino acid lengths ranging from 163—585 aa, protein molecular weights ranging from 18 310 to 64 070, protein isoelectric points ranging from 4.99 to 9.36, and most of subcellular localization in the nucleus. Collinearity analysis showed that C. sativa had 9, 20, and 6 homologous genes with Arabidopsis, tomato and rice, respectively. The predicted results of cis elements indicated that there were more light responsiveness and abscisic acid elements in TCP gene promoter area. The results of gene expression analysis indicated that the expression of C. sativa TCP gene was tissue specific, with higher expression in leaves and flowers. Conclusion This study lays the foundation for in-depth research on the functions of the TCP gene family in C. sativa, further elucidates the possible involvement of TCP genes in various stages of C. sativa growth and development, and provides a theoretical basis for the development of medicinal resources of C. sativa.

中国中医科学院优秀青年科技人才培养专项（ZZ15-YQ-043）；国家自然科学基金青年基金资助项目（82204579）；中国中医科学院中药研究所国家自然科学基金培育专项（ZXKT22010）