目的 采用UPLC-Q-TOF-MS/MS技术解析玉女颗粒进行化学成分。方法 使用Kromasil 100-3.5-C₁₈色谱柱，以乙腈-水梯度洗脱，体积流量1.0 mL/min，柱温30 ℃；使用Kromasil 100-5-C₁₈色谱柱，以甲醇-0.1%甲酸梯度洗脱，体积流量1.0 mL/min，柱温30 ℃；采用电喷雾离子源（ESI），正、负离子模式扫描，根据质谱数据结合文献报道进行成分鉴定。结果 色谱条件①玉女颗粒分析共鉴定出83个化学成分，色谱条件②玉女颗粒分析共鉴定出73个化学成分。2种色谱条件分析鉴定出46个相同化学成分，共推断和鉴别出110个化合物，其中24个化合物经对照品比对得到验证。结论 所建立的针对该方各药味化学成分特点的2种色谱条件能快速、准确地鉴定玉女颗粒中的化学成分，可为经典名方玉女煎物质基础研究和新药研发提供依据，也可为其他中成药的化学成分分析提供参考。

Objective To analyze the chemical composition of Yunv Granules based on UPLC-Q-TOF-MS technology. Methods The separation was carried out on a Kromasil 100-3.5-C₁₈column with a gradient elution of acetonitrile-water at a volume flow rate of 1.0 mL/min and a column temperature of 30 ℃. ESI was used with positive and negative ionization modes, and the composition was identified according to the mass spectrometry data combined with the literature reports. Results A total of 83 chemical components were identified by the analysis of Yunv Granules under chromatographic conditions ①, 73 chemical components were identified by the analysis of Yunv Granules under chromatographic conditions ②, 46 identical chemical components were identified by the analysis of two sets of chromatographic conditions, and a total of 110 chemical components were inferred and identified, of which 24 chemical components were calibrated by the reference substance. Conclusion The two chromatographic conditions established for the characteristics of the chemical composition of each medicinal flavor of the formula can quickly and accurately identify the chemical composition in the Yunv Granules, which can provide a basis for the basic research and research and development of new drugs of the classic famous Yunv decoction, and can also provide a reference for the chemical composition analysis of other proprietary Chinese medicines.

R284.1