目的 以金属有机骨架（metal-organic frameworks，MOFs）为载体，制备桃叶珊瑚苷印迹复合材料，并用于杜仲Eucommiae Cortex中桃叶珊瑚苷的提取和分离。方法 采用溶胶-凝胶法制备了桃叶珊瑚苷印迹聚合物（EM@MIPs），并通过傅里叶变换红外光谱（Fourier transform infrared spectrometer，FT-IR）和扫描电子显微镜（scanning electron microscope，SEM）表征了该聚合物（EM@MIP3）表面化学官能团和形貌特征，通过色谱法考察了印迹材料对杜仲桃叶珊瑚苷的分离效果。结果 印迹材料（EM@MIP3）具有较高的印迹因子（6.830）和较高选择性，相对于喇叭糖苷、车前草苷和京尼平苷，其对桃叶珊瑚苷的选择性因子（α）值分别为4.931、4.896和3.418。采用色谱法测定EM@MIP3柱对桃叶珊瑚苷的吸附能力，其吸附量为51.34 mg/g。使用EM@MIP3柱对杜仲提取物中桃叶珊瑚苷进行在线分离纯化时，桃叶珊瑚苷产品纯度大于85%，回收率高于95%。此外，EM@MIP3聚合物可重复使用。结论 印迹材料（EM@MIP3）有望发展成为一种高效的吸附介质，用于中药中桃叶珊瑚苷的分离和纯化。

Objective To prepare an aucubin imprinted polymer (EM@MIPs) composite with metal-organic frameworks (MOFs) as the carrier and apply its to the extraction and separation of aucubin from Duzhong (Eucommia ulmoides). Methods A sol-gel method was utilized to prepared EM@MIPs and the superficial chemical functional groups and morphology of polymers were characterized by Fourier transform infrared spectrometer (FT-IR) and scanning electron microscope (SEM). The molecular recognition behavior for this imprinted material was evaluated through chromatographic method. Results The imprint materials (EM@MIP3) obtained under the optimized conditions possessed a high imprinting factor (6.830) and a high selectivity toward the template, with selectivity factor (α) values of 4.931, 4.896 and 3.418 relative to bugle glucoside, plantain glucoside and geniposide, respectively. The binding capacity for aucubin on the EM@MIP3 column was determined by frontal chromatography technique, with a value of 51.34 mg/g. The EM@MIP3 column can be utilized to on-line separation of aucubin from the extract of E. ulmoides, obtaining more than 90% purity of aucubin products, with a recovery higher than 95%. In addition, the reusability for the EM@MIP3 polymer was satisfactory. Conclusion This imprint materials (EM@MIP3) are expected to be developed into an efficient adsorption media for the separation and purification of aucubin from Chinese medicines.

国家自然科学基金资助项目（21865011）