目的 研究红果樫木Dysoxylum binectariferum的化学成分及抗肿瘤活性。方法 采用多种色谱方法结合进行化合物分离纯化，根据1D和2D核磁共振及质谱数据鉴定结构。采用MTT法对化合物进行肿瘤细胞增殖毒性评价。结果 从红果樫木叶提取物中得到2个具有由C₂-C₃碳键断裂C₂-C₆碳键闭环产生的双环[3，2，1]辛烷环骨架结构的三萜类化合物，分别为2，3-seco-2，6-cyclo-2a，29-dihydroxytirucalla-7，24-dien-23-oxo-3，9b-olide（1 ）、aphanamgrandiol A（2 ）。化合物1 对人肺癌H1975细胞和人结肠癌HCT116细胞的半数抑制浓度（half inhibitory concentration，IC₅₀）分别为（48.8±2.4）、（28.7±1.6）μmol/L，化合物2 对2种肿瘤细胞的IC₅₀值分别为（44.4±2.1）、（32.5±1.8）μmol/L。结论 化合物1 为新化合物，命名为大叶山楝三萜B。化合物2 首次从樫木属植物中分离得到。化合物1 和2 对肿瘤细胞具有一定的增殖抑制作用。

Objective To study the constituents from Dysoxylum binectariferum and their anti-tumor activities. Methods Various column chromatographic methods were used for chemical constituents isolation. The structures were identified by NMR and MS spectral analysis, and their cytotoxic activities to cancer cells were investigated by MTT method in vitro. Results Two triterpenoid with a bicycle [3,2,1]octane ring skeleton produced by 2,3-ring opening and 2,6-ring closure were obtained, which were identified as 2,3-seco-2,6-cyclo-2a,29-dihydroxytirucalla-7,24-dien-23-oxo-3,9b-olide (1) and aphanamgrandiol A (2). The half inhibitory concentration (IC₅₀) of compound 1 on human lung cancer H1975 cells and human colon cancer HCT116 cells was (48.8 ±2.4) and (28.7 ±1.6) μmol/L, respectively. IC₅₀ of compound 2 on H1975 and HCT116 cells was (44.4 ±2.1) and (32.5 ±1.8) μmol/L. Conclusion Compound 1 is identified as a new compound and named 24(25)-en aphanamgrandiol A and compound 2 is isolated from Dysoxylum for the first time. Compound 1 and 2 exhibited moderate cytotoxicity.

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国家自然科学基金资助项目（32200311）；济南市高校20条（202228020）；齐鲁工业大学（山东省科学院）科教产融合创新试点工程项目（2022PX029）