目的 建立以对照药材为基准物质的定性和不依赖多种对照品定量的白芍Paeoniae Radix Alba药材“质-量”双标控制方法。方法 采用HPLC技术建立白芍对照药材的特征图谱，将其与供试药材图谱进行比对来辨别药材的真伪，即“质”；采用内标物质对特征峰化学成分进行相对定量来区分药材的优劣，即“量”。结果 建立的白芍药材“质-量”分析方法符合方法学考察要求，各供试药材与对照药材的相似度均大于0.90；规定了白芍药材特征峰化学成分相对含量下限。结论 该方法简便、快捷、准确，可以弥补目前白芍药材质量控制的不足，达到全面控制白芍药材的目的。

Objective To establish a "quality-quantity" double standard control method for Baishao (Paeoniae Radix Alba), which is qualitative based on the reference traditional Chinese medicinal materials (TCMM) as standard substances and quantitative without relying on multiple reference substances. Methods HPLC technology was used to establish the characteristic chromatogram of the TCMM of Paeoniae Radix Alba, which was compared with the chromatogram of the tested TCMM to identify the authenticity of the medicinal materials, that is, "quality". The internal standard material was used to distinguish the merits and demerits of medicinal materials by relative quantification of the chemical constituents of characteristic peaks, that is, "quantity". Results The established "quality and quantity" analysis method of Paeoniae Radix Alba met the requirements of methodological investigation, and the similarity between the tested TCMM and the reference TCMM was more than 0.90. The lower limit of the relative content of the chemical components of Paeoniae Radix Alba was specified. Conclusion This method is simple, fast and accurate, which can make up for the shortage of quality control of Paeoniae Radix Alba and achieve the goal of overall control of Paeoniae Radix Alba.

R286.2

辽宁省教育厅创新团队项目（LT2017015）