目的 建立牻牛儿苗Erodium stephanianum的指纹图谱，并同时建立其中4个酚酸类成分含量的一测多评分析（quantitative analysis of multi-components by singlemarker，QAMS）方法，以期对牻牛儿苗药材进行整体质量控制。方法 以没食子酸为参照峰确定共有峰，建立牻牛儿苗的高效液相色谱指纹图谱；以没食子酸为内标，确定柯里拉京、老鹳草素和鞣花酸的相对校正因子并计算含量，实现一测多评。同时采用外标法测定4个成分的含量，比较计算值和实测值的差异。结果 建立了牻牛儿苗的HPLC指纹图谱，10批样品相似度均在0.891以上；以没食子酸为内标建立的柯里拉京、老鹳草素和鞣花酸的相对校正因子分别为0.751、0.214和0.444，重现性良好，10批牻牛儿苗样品QAMS法计算值与实测值无显著差异。结论 所建立的指纹图谱结合QAMS简便可行，可为牻牛儿苗药材整体质量控制提供参考和依据。

Objective To establish the fingerprint of Erodium stephanianum Willd. and a single standard for determination of multiple components method was used to determine the content of four main components simultaneously, in order to control the overall quality of E. stephanianum. Methods Using gallic acid as the reference peak, the common peaks were determined to establish HPLC fingerprint of E. stephanianum. Gallic acid was selected as internal reference and the relative correction factors (RCF) of four components were calculated and the contents were calculated by QAMS. Meanwhile, the contents of four components in E. stephanianum were determined by external standard method. The accuracy and feasibility of QAMS was evaluated by comparing the results between the measured values by external standard method and calculated values by QAMS. Results The HPLC fingerprint of E. stephanianum was established and the similarity of fingerprints of 10 batches of test samples were above 0.891. The RCF of corilagin,geraniin and ellagic acid were 0.751, 0.214 and 0.444 and showed good reproducibility. The contents of four components showed no significant difference in the 10 batches of E. stephanianum determined by external standard method and QAMS. Conclusion The established fingerprint combined with QAMS method are simple and feasible, which can provide some reference and basis for the overall quality control of E. stephanianum.

R286.2

国家自然科学基金项目（81973431）；通化师范学院应用研究项目—中药质量评价研究（01054）