目的 建立大盘龙七Bergeniae Scopulosae Rhizoma的HPLC指纹图谱及多酚类成分的含量测定方法，为大盘龙七药材质量控制及临床应用提供依据。方法 采用Agilent 5 TC-C₁₈色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.1%磷酸水为流动相进行梯度洗脱，体积流量为1.0 mL/min，检测波长为275 nm，柱温25℃；对10批大盘龙七药材进行指纹图谱构建，基于指纹图谱相似度分析结合聚类分析与主成分分析，同时测定熊果苷、没食子酸、岩白菜素、儿茶素、4-O-没食子酰岩白菜素、11-O-没食子酰岩白菜素、儿茶素没食子酸酯的含量。结果 建立了大盘龙七药材的HPLC指纹图谱，其中S5的相似度为0.752，其余批次的相似度均大于0.92，共标定了14个共有峰，指认出7个色谱峰，分别为熊果苷、没食子酸、岩白菜素、儿茶素、4-O-没食子酰岩白菜素、11-O-没食子酰岩白菜素、儿茶素没食子酸酯。聚类分析将10批大盘龙七药材分为3类；经主成分分析，主成分1～4是影响药材样品质量评价的主要因子；采用正交偏最小二乘法判别分析标记了大盘龙七中6个差异性成分。含量测定结果表明10批大盘龙七药材中熊果苷、没食子酸、岩白菜素、儿茶素、4-O-没食子酰岩白菜素、11-O-没食子酰岩白菜素、儿茶素没食子酸酯的含量范围分别为7.30～63.24、0.26～2.98、15.96～78.04、1.54～11.17、0.97～2.54、2.09～7.38、0.64～12.30 mg/g。结论 建立的大盘龙七药材的HPLC指纹图谱及多酚类成分的含量测定方法操作简便、重复性好，可用于大盘龙七药材的定性定量分析，为大盘龙七药材的质量评价提供参考。

Objective To establish HPLC fingerprints of Bergeniae Scopulosae Rhizoma and content determination of phenolic compounds, and provide reference for the quality control and clinical application of Bergeniae Scopulosae Rhizoma. Methods The HPLC separation was carried out by gradual elution with acetonitrile-0.1% phosphoric acid solution. The chromatographic conditions:Agilent 5 TC-C₁₈column (250 mm×4.6 mm, 5 μm), flowing at 1.0 mL/min, the detection wavelength was set at 275 nm and the column temperature was 25 ^°C. The fingerprints of 10 batches of Bergeniae Scopulosae Rhizoma were constructed. Based on the similarity analysis of fingerprints, cluster analysis and principal component analysis, the content of arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin and catechin gallate were determined. Results The HPLC fingerprints of Bergeniae Scopulosae Rhizoma were established, the similarity of S5 was 0.752, and the similarities of other batches were above 0.92. A total of 14 common peaks were demarcated and seven common peaks were identified as arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin and catechin gallate. The 10 batches of Bergeniae Scopulosae Rhizoma were divided into three categories by hierarchical cluster analysis (HCA). According to the principal component analysis (PCA), the principal components 1-4 were the main factors affecting the quality evaluation of the samples. Six differential components in Bergeniae Scopulosae Rhizoma were marked by partial least squares discrimination analysis (OPLS-DA). The contents of arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin and catechin gallate were 7.30-63.24, 0.26-2.98, 15.96-78.04, 1.54-11.17, 0.97-2.54, 2.09-7.38 and 0.64-12.30 mg/g, respectively. Conclusion The established HPLC fingerprints and phenolic compounds determination method are simple and reproducible, which can be used for qualitative and quantitative analysis of Bergeniae Scopulosae Rhizoma and provide reference for quality evaluation of Bergeniae Scopulosae Rhizoma.

R286.2

国家自然科学基金面上项目（81973419）；陕西省重点研发计划一般项目（2022SF-544）；陕西省中医药管理局“双链融合”中青年科研创新团队（2022-SLRH-YQ-003）；陕西省中医药管理局科研项目（2021-ZZ-JC033）；陕西省中医药研究院“苗圃培育计划”项目（2021-05）