目的 基于指纹图谱、指标成分含量探索饮片-基准样品的量值传递关系，采用网络药理学预测温胆汤潜在的质量标志物（quality markers，Q-Marker）。方法 采用HPLC建立温胆汤指纹图谱和指标成分含量测定检测方法，应用网络药理学构建温胆汤“成分-靶点-通路”的网络，对其潜在Q-Marker进行预测。结果 温胆汤基准样品指纹图谱共标定14个共有峰，并指认出6个色谱峰，相似度均>0.90；温胆汤基准样品指标成分含量转移率分别为柚皮苷15.61%～25.15%，橙皮苷5.45%～9.68%；采用网络药理学初步预测温胆汤可能通过调节鞘脂代谢通路、内分泌抵抗、代谢途径等信号通路发挥理气化痰、和胃利胆的功效；根据化合物、靶点及信号通路之间的连接度，初步预测甘草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、甘草酸是温胆汤潜在的Q-Marker。结论 采用指纹图谱、含量测定方法探索温胆汤基准样品量值传递关系，结合网络药理学预测温胆汤治疗疾病的潜在Q-Marker，为温胆汤质量标准的建立及作用机制的研究提供参考。

Objective To explore the quality value transmitting relationship between decoction pieces and substance benchmarks based on the fingerprint and index component content. and predict the potential quality markers (Q-Marker) of Wendan Decoction (温胆汤, WDD) by network pharmacology. Methods HPLC was used to establish the fingerprint and the index components content determination detection methods of WDD, The network pharmacology was used to construct the "component-target-pathway" network relationship of WDD, and its potential Q-Markers were predicted. Results A total of 14 common peaks were calibrated by HPLC fingerprint of WDD. The reference substance identified six characteristic peaks, and the similarity was more than 0.90. The content transfer rate of the WDD benchmark samples were 15.61% to 25.15% for naringin and 5.45% to 9.68% for hesperidin. WDD may exert the effect of regulating qi, resolving phlegm and harmonizing stomach and increasing choleresis regulating sphingolipid signaling pathway, endocrine resistance, metabolic pathways and other signaling pathways predicted by network pharmacology. According to the connectivity between compounds, targets and signaling pathways, it was preliminarily predicted that liquiritin, narirutin, naringin, hesperidin, neohesperidin and glycyrrhizic acid were potential Q-Markers of WDD. Conclusion The characteristic map and the content of index components were introduced to explore the transfer relationship of the standard samples of WDD, and combined with network pharmacology to predict the potential Q-Marker in WDD, which provided a reference for the quality standard establishment and action mechanism research of WDD.

R283.6

国家自然科学基金面上项目（82174209）；广东省中医药局科研项目（20212125）