目的 研究蒙药暖宫七味丸的化学物质组、血中移行成分及药动学，筛选出暖宫七味丸潜在的质量标志物。方法 综合运用UPLC-Triple-TOF-MS/MS、GC-MS技术结合数据库及对照品对比，鉴别暖宫七味丸的化学物质组、血中移行成分；使用UPLC-Q-TRAP-MS/MS研究其体内药动学特征。结果 采用UPLC-Q-TOF-MS/MS鉴别出暖宫七味丸中的76个化学成分，采用GC-MS鉴别出35个化合物。经大鼠ig给药后鉴别出19个吸收进入血中的原型成分，其中，沉香四醇、绿原酸、科罗索酸表现出良好的可测性，且具有明显的药动学特征。结论 沉香四醇、绿原酸、科罗索酸在暖宫七味丸的化学物质组-血中移行成分-药动学研究中具有良好的可测性和可追溯性，可作为暖宫七味丸潜在的质量标志物。

Objective To study the chemical composition, blood migration components and pharmacokinetics and screen the potential quality markers of Nuangong Qiwei Pill (暖宫七味丸), a Mongolian medicine. Methods The UPLC-Triple-TOF-MS/MS and GC-MS technologies were used in combination with the database and the comparison of standard products to identify the chemical composition and blood migration components of Nuangong Qiwei Pill; UPLC-Q-TRAP-MS/MS was used to study its pharmacokinetics in vivo. Results A total of 76 chemical components were identified by UPLC-Q-TOF-MS/MS and 35 compounds were identified by GC-MS. After intragastric administration in rats, 19 prototype components that were absorbed into the blood were identified. Among them, agarotetrol, chlorogenic acid and corosolic acid showed good testability and obvious pharmacokinetic characteristics. Conclusion Agarotetrol, chlorogenic acid and corosolic acid may be potential quality markers of Nuangong Qiwei Pill due to its good measurability and traceability in chemical composition-blood migration component-pharmacokinetics analysis.

R284.1

内蒙古自治区科技计划项目（2020GG0199）；国家重点研发计划项目（2018YFC1708203）