目的 研究连翘Forsythia suspensa的化学成分及其体外抗肿瘤活性。方法 采用多种色谱技术进行分离纯化，通过理化性质和波谱学手段（NMR和MS）鉴定化合物结构，测试各化合物对HepG-2和MCF-7细胞的细胞毒性。结果 从连翘75%乙醇提取物中共分离鉴定出26个化合物，分别为2-(4-羟基苯基)乙基-6-O-[(4-羟基苯基)乙酰基]-β-D-吡喃葡萄糖苷（1）、木通苯乙醇苷B（2）、2-(4-羟基苯基)乙基-6-O-(E)-咖啡酰-β-D-吡喃葡萄糖苷（3）、6'-O-香豆酰-1'-O-[2-(3,4-二羟基苯基)乙基]-β-D-吡喃葡萄糖苷（4）、(+)-松脂素（5）、8-羟基松脂素（6）、松脂素-4-O-β-D-吡喃葡萄糖苷（7）、(+)-表松脂素（8）、(+)-1-羟基-2-表松脂素（9）、连翘脂素（10）、(+)-表松脂素-4'-O-β-D-吡喃葡萄糖苷（11）、(+)-落叶松脂素（12）、(-)-落叶松脂素（13）、rel-(7R,8'R,8S)-forsythialan C（14）、rel-(7R,8'R,8R)-forsythialan C（15）、(7'S,8R,8'R)-异落叶松脂素（16）、罗汉松脂素（17）、罗汉松脂素-4'-O-β-D-吡喃葡萄糖苷（18）、(-)-甘密树皮素B-β-D-吡喃葡萄糖苷（19）、(7R,7'R,8S,8'R)-4,4'-二羟基-3,3'-二甲氧基-7,7'-环氧木脂素-4-O-β-D-吡喃葡萄糖苷（20）、(7R,7'R,8S,8'R)-4,4'-二羟基-3,3'-二甲氧基-7,7'-环氧木脂素-4'-O-β-D-吡喃葡萄糖苷（21）、10-O-β-D-桃金娘烯醇苷（22）、neryl-β-D-glucopyranoside（23）、α-松油醇-8-O-β-D-吡喃葡萄糖苷（24）、连翘环己醇酮（25）和阿魏酸甲酯（26）。结论 化合物1、3、4和19～24、26为首次从连翘属植物中分离得到。在40 μmol/L浓度下化合物5～18对HepG-2和MCF-7细胞系具有一定的细胞毒性活性。

Objective To study the chemical constituents from Lianqiao [Forsythia suspensa (Thunb.) Vahl]. Methods The compounds were separated and purified by various chromatographic techniques, and their structures were identified by physical and chemical properties and spectroscopic methods (NMR and MS). The cytotoxicity of each compound on HepG-2 and MCF-7 cells was tested. Results A total of 26 compounds were isolated and identified as 2-(4-hydroxyphenyl)ethyl-6-O- [(4-hydroxyphenyl)acetyl]-β-D-glucopyranoside (1), calceolarioside B (2), 2-(4-hydroxyphenyl)ethyl-6-O-(E)-caffeoyl-β- D-glucopyranoside (3), 6'-O-coumaroyl-1'-O-[2-(3,4-dihydroxyphenyl) ethyl]-β-D-glucopyranoside (4), (+)-pinoresinol (5), 8-hydroxypinoresinol (6), pinoresinol-4-O-β-D-glucopyranoside (7), (+)-epipinoresinol (8), (+)-1-hydroxy-2-epipinoresinol (9), phillygenin (10), (+)-epipinoresinol-4'-O-β-D-glucopyranoside (11), (+)-lariciresinol (12), (-)-lariciresinol (13), rel- (7R,8′R,8S)-forsythialan C (14), rel-(7R,8′R,8R)-forsythialan C (15), (7'S,8R,8'R)-isolariciresinol (16), matairesinol (17), martairesinol-4'-O-β-D-glucopyranoside (18), (-)-nectandrin B-β-D-glucopyranoside (19), (7R,7′R,8S,8′R)-4,4′-dihydroxy-3,3′- dimethoxy-7,7′-epoxylignan-4-O-β-D-glucopyranoside (20), (7R,7′R,8S,8′R)-4,4′-dihydroxy-3,3′-dimethoxy-7,7′-epoxylignan-4′-O- β-D-glucopyranoside (21), myrtenyl-O-β-D-glucopyranoside (22), neryl-β-D-glucopyranoside (23), α-terpineol-8-O-β-D- glucopyranoside (24), rengyolone (25) and methyl ferulate (26). Conclusion Compounds 1, 3, 4, 19—24 and 26 are separated from the genus of Forsythia for the first time. All compounds are evaluated for cytotoxic activities against MCF-7 and HepG-2 cell lines by MTT assay, and the results showed that compounds 5-18 have potential inhibitory activity on MCF-7 and HepG-2 cell lines at 40 μmol/L.

R284.1

国家自然科学基金青年基金项目（81803696）；西南民族大学中央高校基本科研业务费专项资金项目资助（ZYN2023068）