目的 研究硬尖神香草Hyssopus cuspidatus地上干燥部分的化学成分。方法 采用硅胶柱色谱、高效液相色谱技术等进行分离纯化，通过现代波谱学技术对所得化合物进行结构鉴定，并采用MTT比色法对分离得到的化合物进行体外抑制人胃癌HGC-27细胞的活性筛选。结果 从神香草95%乙醇提取物中分离得到9个化合物，分别鉴定为4S-(2-羟基异丙基)-环己烷-2-烯-1-酮（1）、(3R,4aR,5R,6R)-6-羟基-4a,5-二甲基-3-(丙-1-烯-2-基)-3,4,4a,5,6,7-六氢萘-1(2H)-酮（2）、对茴香酸（3）、三裂鼠尾草素（4）、5-羟基-6,7,3',4'-四甲氧基黄酮（5）、蓟黄素（6）、E-p-香豆醇乙醚（7）、(E)-p-香豆醇γ-O-甲醚（8）、4-羟基-3-甲氧基乙基肉桂酸盐（9）。其中，化合物1为新化合物，命名为神香草酮A。对化合物1～9进行抗肿瘤活性筛选，结果显示，化合物1、3、8对HGC-27胃癌细胞具有一定的抑制作用，其半数抑制浓度（median inhibition concentration，IC₅₀）值分别为8.76、16.34、25.28 μmol/L。结论 化合物1为新化合物，化合物5～9为首次从该属植物中分到。

Objective To study the chemical composition of the above-ground dried parts of Hyssopus cuspidatus Boriss. Methods The constituents were isolated and purified by silica gel column and high performance liquid chromatography techniques repeatedly, and the resulting compounds were structurally characterized by modern wave spectroscopy techniques to determine the structures of the compounds, and the isolated compounds were screened for in vitro inhibitory activity against human gastric cancer HGC-27 cell line using MTT colourimetric assay. Results A total of nine compounds were isolated from the 95% ethanol extract of H. cuspidatus. and were identified as: 4S-(2-hydroxyisopropyl)-cyclohexane-2-en-1-one (1), (3R,4aR,5R,6R)-6-hydroxy-4a,5-dimethyl- 3- (prop-1-en-2-yl)-3,4,4a,5,6,7-hexahydronaphthalen-1(2H)-one (2), p-anisic acid (3), salvigenin (4), 5-hydroxy-6,7,3',4'- tetramethoxy flavone (5), cirsimaritin (6), E-p-coumaryl alcohol ethyl ether (7), (E)-p-coumaryl alcohol γ-O-methyl ether (8), 4-hydroxy-3-methoxy ethyl cinnamate (9). Wherein, compound 1 is a new compound. The antitumor activity screening of compounds 1-9 showed that compounds 1, 3 and 8 had certain inhibitory effects on HGC-27 gastric cancer cells, and their IC₅₀ values were 8.76, 16.34, 25.28 μmol/L, respectively. Conclusion Compound 1 is a new compound. Compounds 5-9 were first isolated from this genus.

R284.1

中国医学科学院医学与健康科技创新工程（2022-I2M-1-017）；新疆维吾尔自治区科技项目计划（2021A03002）