目的 建立经典名方甘草泻心汤（Gancao Xiexin Decoction，GXD）基准样品的HPLC图谱及指标性成分含量测定方法，研究GXD基准样品量值传递规律。方法 制备15批GXD基准样品，建立特征图谱并选用《中药色谱指纹图谱相似度评价》软件进行相似度评价，明确特征峰并对其进行归属。测定指标性成分甘草苷、甘草酸、黄芩苷、盐酸小檗碱、表小檗碱、黄连碱、巴马汀的含量，计算指标成分转移率，分析指标成分在饮片-基准样品中量值传递的规律。结果 15批GXD基准样品指纹图谱相似度均大于0.90，共指认22个共有峰，甘草5个、黄芩5个、甘草和黄芩共有3个、黄连6个、黄芩和黄连共有2个、干姜1个；各指标成分从药材-饮片平均转移率分别为98.69%、98.13%、96.94%、94.59%、91.76%、88.08%、94.16%；饮片-基准样品平均转移率为49.08%、42.24%、28.34%、26.72%、29.32%、33.62%、47.93%。结论 特征图谱与多指标成分含量测定方法相结合，对GXD药材-饮片-基准样品的量值传递过程进行分析研究，为经典名方GXD制剂开发提供参考。

Objective To establish the HPLC profiles and methods for detecting the index components in benchmark sample of the classical recipe Gancao Xiexin Decoction (GXD, 甘草泻心汤) to explicit its law of quantity value transfer. Methods Fifteen batches of GXD substance benchmarks were prepared to establish characterization profiles. The similarity was evaluated by similarity evaluation system for chromatographic fingerprint of TCM, to attribute the characteristic peaks. The the index components of liquiritin, glycyrrhetinic acid, baicalin, berberine hydrochloride, epiberberine, coptisine and palmatine were determined to calculate the transfer rates of the index components and analyze quantitative transfer laws of index components between decoction pieces and samples. Results The similarities of 15 batches of GXD benchmark sample fingerprint profiles were all higher than 0.90. Totally, 22 common peaks were identified, thereinto, five for Gancao (Glycyrrhizae Radix et Rhizoma, GRR), five for Huangqin (Scutellariae Radix, SR), three for GRR and SR, six for Huanglian (Coptidis Rhizoma, CR), two for SR and CR, and one for Ganjiang (Zingiberis Rhizoma). The average transfer rates of herbs to decoction pieces were 98.69%, 98.13%, 96.94%, 94.59%, 91.76%, 88.08%, 94.16% respectively; Average transfer rates of decoction pieces to benchmark sample were 49.08%, 42.24%, 28.34%, 26.72%, 29.32%, 33.62%, 47.93% respectively. Conclusion The research on quantitative transmitting of herbs-decoction pieces-benchmark sample of GXD by combination chromatographic fingerprint and multi-indicator determination method, which can provide references for the the formulation development of classic GXD.

R283.6

吉林省发改委项目“经典名方甘草泻心汤物质基准的建立”（2021C035-4）