目的 建立HPLC特征图谱检测方法，将物性参数及化学成分结合进行相关性分析研究小儿消积止咳口服液（Xiaoer Xiaoji Zhike Oral Liquid，XXZOL）的提取、醇沉、浓缩过程中的量质传递规律，为XXZOL在生产过程中的质量控制提供帮助。方法 分别建立15批XXZOL制备过程中的提取液、醇沉液、浓缩液的特征图谱，并测定15批提取液、醇沉液、浓缩液的物性参数（pH值、电导率、相对密度），结合化学成分含量进行相关性分析，得到在XXZOL制备过程中提取液、醇沉液、浓缩液之间的量质传递规律。结果 建立了15批XXZOL中提取液、醇沉液、浓缩液的特征图谱，标定出13个共有峰，根据对照品指认出6个色谱峰，确定为5-羟甲基糠醛（峰2）、绿原酸（峰4）、连翘酯苷A（峰8）、芸香柚皮苷（峰9）、橙皮苷（峰10）、新橙皮苷（峰11）；经主成分分析（principal component analysis，PCA），提取出5个主成分，累积方差贡献率为91.3%；量质传递规律研究发现6个成分在提取液-醇沉液中的转移率较低，在醇沉液-浓缩液中的转移率较高。根据正交偏最小二乘-判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA）筛选得到5个差异指标；根据测定的物性参数与化学成分相关性分析，醇沉液与浓缩液中相对密度与芸香柚皮苷的含量都呈显著正相关，并且连翘酯苷A的含量与pH值呈显著负相关，提取液与浓缩液中新橙皮苷含量与连翘酯苷A含量都呈显著负相关。5-羟甲基糠醛、绿原酸、连翘酯苷A、芸香柚皮苷、新橙皮苷在提取液、醇沉液、浓缩液之间都呈显著正相关，橙皮苷含量在醇沉液和浓缩液之间呈显著正相关。结论 得到的XXZOL的提取、醇沉、浓缩过程的量质传递规律，可为XXZOL在生产过程中的质量控制提供帮助。

Objective To establish a HPLC characteristic chromatogram detection method, the physical parameters and chemical composition were combined for correlation analysis to study the mass transfer law of the extraction, alcohol precipitation and concentration process of Xiaoer Xiaoji Zhike Oral Liquid (小儿消积止咳口服液, XXZOL), so as to provide help for the quality control of XXZOL in the production process. Methods The characteristic chromatograms of extract, alcohol precipitate solution and concentrated liquid in the preparation process of 15 batches of XXZOL were established, and the physical parameters (pH value, conductivity, relative density) of 15 batches of extract, alcohol precipitate solution and concentrated liquid were determined. The correlation analysis was carried out by combining the content of chemical components, and the mass transfer law between extract, alcohol precipitate solution and concentrated liquid in the preparation process of XXZOL was obtained. Results The characteristic chromatograms of the extract, alcohol precipitation solution and concentrated solution of 15 batches of XXZOL were established, and 13 common peaks were identified. According to the reference substance, six chromatographic peaks were identified as 5-hydroxymethylfurfural, chlorogenic acid, forsythoside A, narirutin, hesperidin and neohesperidin. Five principal components were extracted by principal component analysis (PCA), and the cumulative variance contribution rate was 91.3%. The study of mass transfer law found that the transfer rate of six components in water extract-alcohol precipitate was low, and the transfer rate in alcohol precipitate-concentrated solution was high. Five differential indexes were screened according to orthogonal partial least squares-discriminant analysis (OPLS-DA). According to the correlation analysis between the measured physical parameters and the chemical composition, the relative density in the alcohol precipitation solution and the concentrated solution was significantly positively correlated with the content of narirutin, and the content of forsythoside A was significantly negatively correlated with pH value. The content of neohesperidin in the extract and concentrated solution was significantly negatively correlated with the content of forsythoside A. There was a significant positive correlation between 5-hydroxymethylfurfural, chlorogenic acid, forsythoside A, narirutin and neohesperidin in the extract, alcohol precipitation and concentrated liquid. The content of hesperidin was significantly positively correlated between alcohol precipitation and concentrated liquid. Conclusion The quality transfer law of the extraction, alcohol precipitation and concentration process of XXZOL obtained in this study can provide help for the quality control of XXZOL in the production process.

R283.6

国家重点研发计划（2019YFC1711204）；陕西省教育厅项目（21JC011）；陕西省中医药管理局（2021-04-ZZ-007）