目的 研究裸花紫珠Callicarpa nudiflora叶的化学成分，并对分离的化合物进行初步的抗炎筛选。方法 采用各种色谱方法（硅胶、MCI、羟丙基葡聚糖凝胶、ODS）进行分离纯化，并结合核磁、质谱数据明确其结构。通过Griess法对分离得到的化合物进行初步的抗炎活性筛选。结果 从裸花紫珠叶中醋酸乙酯部位分离得到17个单体化合物，分别鉴定为(6S,7R)-3-oxo-megastigma-4,8-dien-7-O-β-D-glucoside（1）、phoebenoside A（2）、(6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside（3）、blumenol C glucoside（4）、异毛蕊花糖苷（5）、myricoside（6）、肉苁蓉苷D（7）、地黄苷（8）、木通苯乙醇苷B（9）、4,4'-dimethoxy-3'-hydroxy-7,9':7',9-diepoxylignan-3-O-β-D-glucopyranoside（10）、木犀草素-7-O-葡萄糖苷（11）、木犀草素- 7-O-β-D-葡萄糖苷（12）、木犀草素-4′-O-β-D-葡萄糖苷（13）、大波斯菊苷（14）、luteolin-7-O-β-L-rhamnopyranosyl (1→2)-β-D- glucopyranoside（15）、芹菜素-7-O-β-D-新橙皮苷（16）、2-O-butyl-1-O-(2′-ethylhexyl) benzene-1,8-dicarboxylate（17）。化合物7、14对NO生成抑制率达到45%以上。结论 化合物1～4、9～11、17为首次从该属植物中分离获得。其中化合物7和14能显著抑制NO的产生，具有良好的体外抗炎效果。

Obective To study of the chemical composition of the ethyl acetate parts of Callicarpa nudiflora, and preliminary screen out the isolated compounds with anti-inflammatory activities. Methods Various chromatographic methods (silica gel, MCI, hydroxypropyl dextran gel, ODS) were used for the isolation and purification, and the structure was clarified by combination with NMR and MS data. The isolated compoundswere screened for preliminary anti-inflammatory activity by the Griess method. Results Seventeen monomeric compounds were isolated from C. nudiflora, which were identified as (6S,7R)-3-oxo-megastigma- 4,8-dien-7-O-β-D-glucoside (1), phoebenoside A (2), (6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside (3), blumenol C glucoside(4), isoacteoside (5), myricoside (6), cistanoside D (7), martynoside (8), calceolarioside B (9), 4,4'-dimethoxy-3'-hydroxy-7,9':7',9- diepoxylignan-3-O-β-D-glucopyranoside (10), luteolin-7-O-glucuronide (11), luteolin-7-O-β-D-glucoside (12), luteolin-4′-O-β-D- glucoside (13), apigenin-7-O-glucoside (14), luteolin-7-O-β-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside (15), apigenin-7-O- β-D-neohesperidoside (16), 2-O-butyl-1-O-(2′-ethylhexyl) benzene-1,8-dicarboxylate (17). Compounds 7 and 14 inhibited NO production by more than 45%. Conclusion Compounds 1—4, 9—11 and 17 are the first isolated from this genus. Among them, compounds 7 and 14 significantly inhibited NO production and had good in vitro anti-inflammatory effects.

R284.1

江西省重点研发计划项目（20212BBG71007）