目的 研究奇楠沉香Aquilaria sinensis中具有神经细胞损伤保护作用的活性化合物。方法 利用硅胶柱色谱、半制备液相色谱等现代色谱技术进行分离纯化，结合核磁共振和质谱等现代谱学手段鉴定其结构，同时体外法测定化合物对皮质酮（corticosterone）诱导PC12细胞损伤的保护作用。结果 从奇楠沉香超临界提取物中分离得到2个桉烷型倍半萜类化合物，分别鉴定为 (7R,8S,10R)-(+)-8-hydroxy-selina-4,11-dien-12,15-dial（1）和 (7S,8S,10R)-(+)-8,11-dihydroxy-selina-4-en-15-al（2）；体外对PC12细胞损伤存活率显著升高。结论 化合物1和2为新化合物，分别命名为奇楠醇A和奇楠醇B，均对皮质酮诱导PC12细胞损伤具有一定的保护作用。

Objective To investigate the neuroprotective of active molecules in “Qi-Nan” agarwood from Aquilaria sinensis. Methods The compounds were isolated and purified by modern chromatography techniques, such as silica gel column chromatography, and semi-preparative high performance liquid chromatography, and the chemical structures were identified by NMR and MS spectrometry. The neuroprotective effect of these compounds on corticosterone (CORT) induced PC12 cell damage was evaluated in vitro. Results Two eudesmane-type sesquiterpenoids were isolated from the supercritical extract of “Qi-Nan” agarwood, and named as (7R,8S,10R)-(+)-8-hydroxy-selina-4,11-dien-12,15-dial (1) and (7S,8S,10R)-(+)-8,11-dihydroxy- selina-4-en-15-al (2). Compounds 1 and 2 significantly increased the survival rate of PC12 cells after injury in vitro. Conclusion Compounds 1 and 2 are new compounds with the trivial name aquqinanols A and B, which have moderate neuroprotective effects against PC12 cell damage induced by CORT.

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海南省重点研发计划项目（ZDYF2020111）；海南省重点研发计划项目（ZDYF2022SHFZ030）；中国医学科学院医学与健康科技创新工程（2021-I2M-1-032）；国家自然科学青年基金项目（82204657）