目的 探究胃癌患者的基底膜差异基因之间以及与预后的相关性，并预测调控差异基因表达的中药，通过统计分析中药的性味归经、药物分类为中医药治疗胃癌提供理论支持，有利于后续中医药开发及中西医联合治疗策略的制定。方法 下载TCGA数据库中胃癌患者的转录组数据，通过检索文献获取基底膜相关基因并筛选、分析其在胃癌的表达量及共表达情况，并对差异基因进行基因本体（gene ontology，GO）功能富集分析、京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）通路富集分析和疾病本体（disease ontology，DO）富集分析。通过GEPIA数据库对差异倍数大的基底膜差异基因进行预后分析，再通过CTD数据库查找对基因有调控作用的化学成分。应用COREMINE数据库查找相关中药，并通过古今医案云平台统计高频中药的性味归经、药物分类。结果 共筛选出15个与预后相关的基底膜基因，根据参考文献筛选出45种化学成分，得到高频中药为茶树根、姜皮、干姜、丹参、人参等；药性以平、温为主；药味以甘、苦为主；归经以脾、肺、肝经居多；以补虚药、收涩药、清热药、活血化瘀药、理气药、化痰止咳平喘药为主。结论 通过生物信息学分析得出胃癌相关基底膜基因及对应的高频中药，为治疗胃癌的中药新药开发及中医药理论的微观挖掘提供思路。

Objective To explore the correlation between basement membrane differential genes in patients with stomach adenocarcinoma and the correlation between the differential genes and prognosis, and predict the traditional Chinese medicine (TCM) that regulates the expression of differential genes, and provide theoretical support for the treatment of gastric cancer by TCM through statistical analysis of the property, flavor, attributive channel and drug classification of TCM, which is conducive to the subsequent development of TCM and the formulation of treatment strategies of integrated Chinese and western medicine. Methods Transcriptome data of patients with stomach adenocarcinoma were downloaded from the TCGA database. The basement membrane-related genes were obtained through literature search, and their expression levels and co-expression in gastric cancer were screened and analyzed, and the differential genes were analyzed through gene ontology (GO) functional enrichment analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis and disease ontology (DO) enrichment analysis. Prognostic analysis of basement membrane differential genes with large differential multiples was performed using GEPIA database. CTD database was used to find the chemical components that have regulatory effects on genes. COREMINE database was used to search for related TCM, and property, flavor, attributive channel and drug classification of high-frequency TCMs were counted through the cloud platform of ancient and modern medical records. Results A total of 15 basement membrane genes related to prognosis were selected, 45 chemical components were screened according to the references, and the high frequency TCMs were Chashugen (root of Camellia sinensis), Jiangpi (peel of Zingiber officinale), Ganjiang (Zingiberis Rhizoma), Danshen (Salviae Miltiorrhizae Radix et Rhizoma), Renshen (Ginseng Radix et Rhizoma), etc. The medicinal properties were mainly flat and warm; The flavor were mainly sweet and bitter; Spleen, lung and liver channels were the main channels. The main drugs were tonifying deficiency, receiving astringent, clearing heat, promoting blood circulation and removing blood stasis, regulating qi, resolving phlegm and relieving cough and asthma. Conclusion The basement membrane genes related to stomach adenocarcinoma and the corresponding high frequency TCM were obtained through the analysis of bioinformatics technology, which provides ideas for the development of new Chinese medicine for the treatment of stomach adenocarcinoma and the micro-excavation of Chinese medicine theory.

R285

广东省中医药局科研项目（20231114）