目的 采用AHP-TOPSIS综合评价法筛选三七Notoginseng Radix et Rhizoma生药粉最佳灭菌工艺。方法 以三七皂苷R₁、人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁、人参皂苷Rd总量和抗氧化活性、综合外观性状以及灭菌率的综合评分为指标，在单因素实验的基础上，分别对比干热灭菌、湿热灭菌、紫外灭菌、新型辐照灭菌法（电子加速器产生的能量低于10 MeV的电子束）4种灭菌方法对三七生药粉灭菌前后灭菌效果和质量的影响。结果 根据AHP-TOPSIS综合评价结果排序可知，4类灭菌方式对三七生药粉质量影响的优劣顺序为新型辐照灭菌>干热灭菌>湿热灭菌>紫外灭菌。其中新型辐照灭菌在剂量6 kGy及以上能完全杀灭三七生药粉中的微生物同时较好地保留了三七生药粉中的有效成分，综合考虑灭菌操作便利性、经济效益性和安全性，确定三七生药粉的最佳灭菌方法为新型辐照灭菌，剂量为6 kGy。结论 新型辐照灭菌法与其他灭菌方法相比，在保证灭菌效果的同时能更大程度地保留三七生药粉中的有效成分，能低温度、短时间、相对安全地得到符合规定的三七生药粉，且优选得到的灭菌工艺稳定可行、重复性好，可为其后续工艺开发和工业化生产提供参考。

Objective To screen the optimal sterilization process of Sanqi (Notoginseng Radix et Rhizoma, NRR) raw powder using AHP-TOPSIS comprehensive evaluation method. Methods The chemical components such as notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁, ginsenoside Rd total content, antioxidant capacity of NRR, comprehensive appearance traits and sterilization rate were selected as evaluation indexes, on the basis of single factor test, four sterilization methods, namely dry heat sterilization, wet heat sterilization, ultraviolet sterilization and new irradiation sterilization (electron beam with energy less than 10 MeV produced by electron accelerator), were respectively compared on the sterilization effect and quality of NRR raw powder before and after sterilization. Results According to the AHP-TOPSIS comprehensive evaluation results, the effect of four sterilization methods on the quality of NRR raw powder was in the order of new irradiation sterilization > dry heat sterilization > moist heat sterilization > ultraviolet sterilization. The new irradiation sterilization at doses of 6 kGy and above can completely kill the microorganisms while better retaining the active ingredients in the raw NRR raw powder, and considering the convenience of sterilization operation, economic efficiency and safety, the best sterilization method for NRR raw powder is the new irradiation sterilization at a dose of 6 kGy. Conclusion Compared with other sterilization methods, the new irradiation sterilization method can obtain the prescribed raw NRR powder in low temperature, short time and relatively safely, and the optimized sterilization process is stable, feasible and reproducible, which can provide a reference for the subsequent process development and industrial production.

R283.6

国家中医药管理局中药炮制技术传承基地建设项目；湖南省自然科学基金资助项目（2020JJ9007）；湖南省自然科学基金资助项目（2021JJ80066）；湖南省中药炮制技术传承基地（zjzx-001）；湖南中医药大学校级基金（2019XJJJ053）