目的 应用正交试验设计，结合基准关联度和层次分析法-熵权法优化经典名方黄连汤的提取工艺。方法 以黄连碱、巴马汀、小檗碱、6-姜辣素、甘草苷、甘草酸、指纹图谱相似度及干膏率为关键质量属性，以加水量、浸泡时间、提取时间为关键工艺参数，采用单因素确定各因素水平，正交试验设计优化工艺参数。计算不同提取参数的各评价指标下各样品与基准样品的基准关联度，采用AHP-熵权法确定各评价指标的综合权重系数，进行综合评分。结果 正交试验设计联用基准关联度和AHP-熵权法建立的工艺评价函数模型为综合评分＝相对偏差（relative deviation，SR）_{i，黄连碱}×30%＋SR_{i，巴马汀}×24%＋SR_{i，小檗碱}×28%＋SR_{i，6-姜辣素}×5%＋SR_{i，甘草苷}×5%＋SR_{i，甘草酸}×6%＋SR_{i，干膏率}×2%，该模型稳定可靠，确定提取工艺为全方饮片加6倍量水，浸泡60 min，煎煮45 min。验证实验综合评分均值为90.11，RSD为1.09%，符合基准样品的要求，且方法具有适用性。结论 筛选的黄连汤现代提取工艺参数具有可行性，可为其他经典名方的开发利用提供理论依据。

Objective The extraction process of the classical prescription Huanglian Decoction (黄连汤) was optimized based on orthogonal test, standard relation and analytic hierarchy process combined with entropy method. Methods With the contents of coptisine, palmatine, berberine, 6-gingerol, liquiritin, glycyrrhizic acid, fingerprint similarity, paste-forming rate as critical quality attributes (CQAs). The amount of water, soaking time and extraction time were used as the critical process parameters (CPPs). The single factor experiment design was used to determine the level of each factor, and the orthogonal test was used to optimize the process parameters. The standard relation between each samples and standard decoction of various indexes of different extraction parameters was calculated, while AHP-entropy method was applied to determine the weight of each index for comprehensive score. Results A process evaluation function model was established by using orthogonal test combined with standard relation and AHP-entropy method as follow:comprehensive score=SR_i,coptisine×30% + SR_i,palmatine×24% + SR_i,berberine×28% + SR_i,6-gingerol×5% + SR_i,liquiritin×5% + SR_{i,glycyrrhizic acid}×6% + SR_{i,paste-forming rate}×2%. The function model was stable and reliable. The extraction process was to add six times of water to the whole decoction pieces, soak for 60 min and extract for 45 min. The average score of the validation test was 90.11, and the RSD was 1.09%, which met the requirements of the reference material standard, and the method was applicable. Conclusion The modern extraction parameters of Huanglian Decoction screened by this method were feasible, which provided a theoretical basis for the development and utilization of other classic prescriptions.

R283.6

国家重点研发计划项目（2018YFC1707000）；江苏省研究生科研创新计划项目（KYCX23-2027）