目的 探讨复方野菊花提取物对肝火亢盛型高血压大鼠模型的影响及降压作用机制。方法 自发性高血压大鼠（spontaneously hypertensive rats，SHR）ig复方附子汤建立肝火亢盛型高血压大鼠模型，给予复方野菊花提取物干预后，检测大鼠中医证候指标、心率和血压，测定血清与肾脏中肾素-血管紧张素-醛固酮系统（renin-angiotensin-aldosterone dystem，RAAS）相关因子的含量，检测血清中一氧化氮（nitric oxide，NO）、内皮素-1（endothelin-1，ET-1）及血脂水平，观察靶器官组织形态学变化。结果 复方野菊花提取物能显著降低模型大鼠眩晕恢复时间、面温、耳温、尿液吸光度（P<0.01），增加唾液分泌量及粪便含水量（P<0.01），缓解模型大鼠尖叫、攻击、打斗、撕咬等攻击性行为；显著降低模型大鼠心率、收缩压、舒张压和平均动脉压（P<0.05、0.01）；显著降低血清总胆固醇（total cholesterol，TC）、三酰甘油（triacylglycerol，TG）、低密度脂蛋白胆固醇（low density lipoprotein-cholesterol，LDL-C）和ET-1水平（P<0.01），升高NO水平（P<0.01）；改善心脏、肾脏及胸主动脉组织结构异常；降低血清中醛固酮（aldosterone，ALD）、肾素、血管紧张素-II（angiotensin-II，Ang-II）、血管紧张素转换酶（angiotensin converting enzyme，ACE）水平（P<0.05、0.01），降低肾脏肾素、Ang-II、ACE和胸主动脉Ang-II的蛋白表达（P<0.05、0.01），增加肾脏ACE2蛋白表达（P<0.01）。结论 复方野菊花提取物能够缓解高血压模型大鼠肝火亢盛证，改善模型大鼠的内皮状态，其降压机制可能与抑制RAAS过度激活有关。

Objective To explore the effect and hypotensive mechanism of compound Dendranthema indicum extract on hypertensive model rats with liver-fire hyperactivity syndrome. Methods Spontaneously hypertensive rats (SHR) were ig compound Fuzi Decoction (复方附子汤) to induce hypertensive model with liver-fire hyperactivity syndrome, after the intervention of compound D. indicum extract, the indexes of TCM syndromes, heart rate and blood pressure of rats were monitored, the contents of renin-angiotensin-aldosterone dystem (RAAS) related factors in serum and kidney were measured, the levels of nitric oxide (NO), endothelin-1 (ET-1) and blood lipids in serum were detected, and the histomorphological changes of kidney, heart and aorta were observed. Results Compound D. indicum extract could significantly reduce the recovery time of vertigo, face temperature, ear temperature and urine absorbance (P < 0.01), increase saliva secretion and fecal water content (P < 0.01), and alleviate the temperament changes of model rats such as screaming, fighting and biting. Compound D. indicum extract significantly decrease heart rate, systolic blood pressure, diastolic blood pressure and mean arterial pressure (P < 0.05, 0.01), reduce total cholesterol (TC), triacylglycerol (TG), low density lipoprotein-cholesterol (LDL-C) and ET-1 levels in serum (P < 0.01), while increase NO level (P < 0.01). Compound D. indicum extract could improve the abnormal tissue structure of heart, kidney and thoracic aorta, reduce the levels of aldosterone (ALD), renin, angiotensin-II (Ang-II) and angiotensin converting enzyme (ACE) in serum (P < 0.05, 0.01), reduce the protein expressions of renin, Ang-Ⅱ, ACE in kidney and Ang-Ⅱ in thoracic aorta (P < 0.05, 0.01), increase the protein expression of ACE2 in kidney (P < 0.01). Conclusion Compound D. indicum extract can alleviate liver-fire hyperactivity syndrome in hypertensive model rats, improve the endothelial status of hypertensive model rats, and its hypotensive mechanism may be related to the inhibition of RAAS over activation.

R285.5

国家自然科学基金资助项目（81873036）；国家自然科学基金资助项目（82003977）；浙江省重点研发计划项目（2017C03052，2020C04020）；浙江省重点实验室项目（2012E10002）