目的 对倒心盾翅藤Aspidopterys obcordate藤茎的化学成分进行研究。方法 采用正相硅胶、反相ODS、MCI gel CHP 20P等柱色谱及制备高效液相色谱等分离方法进行分离纯化，运用旋光、核磁共振、质谱和圆二色光谱等多种波谱学技术鉴定化合物的化学结构；并采用一水草酸钙结晶（COM）诱导的大鼠肾小管上皮NRK-52E细胞损伤模型，对分离得到的单体成分进行活性筛选，初步探讨各单体成分对COM致NRK-52E细胞损伤的保护作用。结果 从倒心盾翅藤藤茎70%乙醇提取物中分离得到17个化合物，分别鉴定为槲皮素（1）、(＋)-儿茶素（2）、异黄烷香豆素（3）、龙脑苷（4）、大柱香波龙烷-9-O-β-D-葡萄糖苷（5）、长春花糖苷Ⅱ（6）、2,3-seco-sonderianol（7）、木栓酮（8）、2α,3β,23-三羟基-12-烯-28-齐墩果酸（9）、(24S)-24-乙基胆甾-3β,5α,6β-三醇（10）、辛二酸单甲酯（11）、1-壬二酸甘油酯（12）、(9S,10E,12S,13S)-9,12,13-三羟基-10-十八碳烯酸（13）、(9S,10E,12S,13S,15Z)-三羟基十八烷-10,15-二烯酸（14）、异丙醇-[6-O-(β-D-木糖基)]-β-D-吡喃葡萄糖苷（15）、3,4-二羟基苯甲酰胺（16）和4'-O-(β-D-吡喃葡萄糖基)-维生素B5（17）。结论 化合物3～5、7、9～17共13个化合物为首次从盾翅藤属中分离得到；并首次报道了化合物17的¹³C-NMR谱数据。其中，化合物1～3、10、13和14在不影响细胞活力的情况下，对COM所致NRK-52E细胞的损伤具有明显的抑制作用。

Objective To study the chemical constituents of the 70% EtOH extract of Aspidopterys obcordate vines. Methods Silica gel, ODS, and MCI gel CHP 20P column chromatographies, as well as preparative high-performance liquid chromatography were applied for the constituents' isolation from the 70% EtOH extract. Then,[α]_D, NMR, MS, CD, and other related spectroscopic analyses were used to identify their structures. Moreover, the protective effects of each purified compound on calcium oxalate monohydrate (COM) induced rat renal tubular epithelial cells (NRK-52E cells) injury were investigated. Results A total of 17 compounds, quercetin (1), (+)-catechin (2), isoflavanocoumarin (3), 1-borneol-β-D-apisyl-β-D-glucopyranoside (4), lauroside B (5), roseoside II (6), 2,3-seco-sonderianol (7), friedelin (8), 2α,3β,23-trihydroxyolean-12-en-28-oic acid (9), (24S)-24-ethylcholesta-3β,5α,6β-triol (10), suberic acid monomethyl ester (11), 1-azeloyl-rac-glycer (12), (9S,10E,12S,13S)-9,12,13-trihydroxy-10-octadecenoic acid (13), fulgidic acid (14), 2-[6-O-(β-D-xylopyranosyl)-β-D-glucopyranosyloxy]propane (15), 3,4-dihydroxybenzamide (16), and 4'-O-(β-D-glucopyranosyl)-DL-pantothenic acid (17) were obtained from the 70% EtOH extract of A. obcordate vines. Conclusion Thirteen compounds including 3-5, 7, and 9-17 were isolated firstly from Aspidopterys genus. Meanwhile, the ¹³C-NMR data of compound 17 was reported for the first time. And compounds 1-3, 10, 13, and 14 could significantly improve the survival rate of COM-induced NRK-52E cells injury, suggesting that they had some protective effect against the cells damage.

R284.1

国家自然科学基金面上项目（82074118）