[关键词]
[摘要]
目的 对洋紫荆Bauhinia variegate的化学成分进行研究。方法 综合利用Sephadex LH-20、Chromatorex C18、Diaion HP20SS等柱色谱分离技术进行分离纯化,并综合应用HR-ESI-MS、NMR、ECD等多种波谱方法鉴定化合物结构。结果 从洋紫荆花的甲醇提取物中分离得到8个化合物,分别鉴定为4,6-二羟基亚环己基乙腈-8-β-D-葡萄糖苷(1)、L-色氨酸(2)、咖啡酸甲酯(3)、对羟基苯甲酸β-D-吡喃葡萄糖酯苷(4)、山柰酚-3-O-α-L-鼠李糖苷(5)、山柰酚-3-O-β-芸香苷(6)、山柰酚-5-O-[α-L-鼠李糖基-(1→6)-β-D-吡喃葡萄糖苷](7)、kaempferol 3-O-β-glucopyranosyl (1→2)[α-rhamnopyranosyl(1→6)]-β-glucopyranoside(8)。结论 化合物1为新化合物,命名为蝙蝠葛氰苷G,化合物2~6、8为首次从洋紫荆中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents of Bauhinia variegate. Methods The methanol extract of flowers of B. variegate was isolated and purified by column chromatographies with Sephadex LH-20, Chromatorex C18, Diaion HP20SS. And their structures were identified by various spectroscopic methods, such as HR-ESI-MS, NMR, ECD, et al. Results A total of eight compounds were isolated from the extract of flowers of B. variegate. Their structures were identified as 4,6-dihydroxycyclohexylideneacetonitrile-8-β-D-glucoside (1), L-tryptophan (2), methyl caffeate (3), p-hydroxybenzoyl-β-D-glucopyranoside (4), kaempferol-3-O-α-L-rhamnoside (5), kaempferol-3-O-β-rutidine (6), kaempferol-5-O-[α-L-rhamnol-(1→6)-β-D-glucuronoside] (7), kaempferol 3-O-β-glucopyranosyl(1→2)[α-rhamnopyranosyl(1→6)]-β-glucopyranoside (8). Conclusion Compound 1 is a new compound named menisdaurin G. Compounds 2-6 and 8 were obtained from the plant for the first time.
[中图分类号]
R284.1
[基金项目]
柳州市科技攻关项目(2019B0801b006);国家自然科学基金项目(82060764);广西科学院基本科研业务费项目(CQZ-C-1901);桂林市创新平台与人才计划项目(20210102-3)