目的 研究黑柳Salix nigra树皮的化学成分，并对部分化合物进行抑制脂肪生成活性研究。方法 采用多种色谱分离技术进行分离和纯化，并运用核磁共振波谱技术鉴定其结构。以秀丽隐杆线虫Caenorhabditis elegans为实验模型，利用油红O和苏丹黑B染色，研究从黑柳树皮中分离到的部分化合物对秀丽隐杆线虫脂肪生成的影响。结果 从黑柳树皮甲醇提取物的正丁醇萃取部位中共分离得到12个化合物，分别鉴定为柳皮苷（1）、特里杨苷（2）、7-水杨酰-2'-苯甲酰水杨苷（3）、cochinchiside A（4）、2'-苯甲酰水杨苷（5）、胡萝卜苷（6）、rosin（7）、水杨苷（8）、chalcononaringenin-2'-O-β-D-glucopyranoside（9）、3,4-二羟基苯甲酸（10）、苯甲酸（11）和水杨酸（12）。其中化合物1、2、5和9能够显著抑制秀丽隐杆线虫脂肪生成。结论 12个化合物均为首次从黑柳中分离得到。化合物5对秀丽隐杆线虫脂肪生成的抑制作用最强。

Objective To study the chemical composition from the barks of Salix nigra Marshall and its anti-adipogenic activity. Methods The chemical constituents from the barks of S. nigra were isolated by chromatographic methods and the structures of the isolated compounds were elucidated on the basis of NMR spectroscopic analyses. The anti-adipogenic activity of some compounds was evaluated in Caenorhabditis elegans by oil red O and Sudan black B staining methods. Results A total of 12 compounds were isolated from barks of S. nigra and identified as salicortin (1), tremulacin (2), salicyloyltremuloidin (3), cochinchiside A (4), tremuloidin (5), daucosterol (6), rosin (7), salicin (8), chalcononaringenin-2'-O-β-D-glucopyranoside (9), 3,4-dihydroxybenzoic acid (10), benzoic acid (11), and salicylic acid (12), respectively. Among them, compounds 1, 2,5 and 9 could significantly reduce adipose accumulation in C. elegans. Conclusion All twelve compounds were isolated from S. nigra for the first time. Compound 5 exhibited the strongest effect to reduce adipose accumulation in C. elegans.

大学生创新创业训练计划项目（S202210656135）