目的 采用指纹图谱、多成分含量测定结合化学计量学方法分析和比较不同基原白芷药材（白芷Angelica dahurica和杭白芷Angelica dahurica var.formosana）的化学成分，为白芷药材的质量控制提供技术方法及数据支撑。方法 采用高效液相色谱法对白芷和杭白芷药材化学成分进行分析，建立不同基原白芷药材化学指纹图谱；在此基础上，对白芷和杭白芷药材中的花椒毒酚、水合氧化前胡内酯、白当归素、花椒毒素、佛手柑内酯、氧化前胡素、欧前胡素、珊瑚菜素和异欧前胡素9种香豆素类成分进行含量测定；进一步应用聚类分析、主成分分析及偏最小二乘判别分析等化学计量学方法对白芷与杭白芷进行区分与比较，寻找差异化合物。结果 建立了不同基原白芷药材的化学指纹图谱，共标定了11个共有峰，所建立的化学指纹图谱专属性良好，可用于白芷药材的质量评价；多成分含量测定和化学计量学结果表明，白芷和杭白芷药材中所含香豆素类成分种类无差异，但香豆素类成分含量有明显差异；佛手柑内酯、珊瑚菜素、异欧前胡素、氧化前胡素和花椒毒素为白芷和杭白芷药材间的差异性化合物，可以作为区分和鉴别二者的质量标志物。结论 基于指纹图谱、多成分含量测定和化学计量学方法，明确了白芷和杭白芷药材化学成分差异，为不同基原白芷药材的质量控制和基原鉴定提供了分析方法和科学依据。

Objective To compare the chemical composition and find the chemical makers for quality evaluation of Angelica dahurica and Angelica dahurica var. formosana by fingerprint, multi-component determination and chemometrics, so as to provide technical methods and data support for the quality control of Baizhi (Angelicae Dahuricae Radix). Methods In this present study, an accurate and reliable fingerprint of Angelicae Dahuricae Radix was established based on HPLC. Furthermore, nine bioactive coumarins, including xanthotoxol, oxypeucedanin hydrate, byakangelicin, xanthotoxin, bergapten, oxypeucedanin, imperatorin, phellopterin and isoimperatorin in A. dahurica and A. dahurica var. formosana samples were simultaneously determined. Moreover, chemometrics method, such as hierarchical clustering analysis, principal component analysis and partial least squares discriminant analysis were performed to compare and discriminate A. dahurica and A. dahurica var. formosana samples, and explore the chemical markes for quality evaluation of Angelicae Dahuricae Radix. Results The specific fingerprints of Angelicae Dahuricae Radix were obtained, and a total of 11 common peaks were marked. The established fingerprint method could be used for quality control of Angelicae Dahuricae Radix. The quantitative and chemometrics analysis results indicated the contents of bioactive coumarins were notably different between A. dahurica and A. dahurica var. formosana samples, and five coumarins including bergapten, phellopterin, isoimperatorin, oxypeucedanin and xanthotoxin were selected as quality markers for responsible for the significant differences between A. dahurica and A. dahurica var. formosana, which could be used for the quality evaluation of Angelicae Dahuricae Radix. Conclusion The differences of chemical components of A. dahurica and A. dahurica var. formosana were determined based on fingerprint, multi-component determination and chemometrics, which might provide detailed information and acceptable analysis methods for the identification of botanical origins and quality evaluation of Angelicae Dahuricae Radix.

R286.2

河北省自然科学基金资助项目（H2021423004）；河北省中医药管理局科研计划项目（Z2022019，2022100）；河北省第二期现代农业产业技术体系创新团队项目（HBCT2018060205）；第四次全国中药资源普查（Z135080000022）；河北省省级科技计划（223777127D）；中央引导地方科技发展资金项目（226Z7714G）