目的 研究莱菔子Raphani Semen的化学成分及其调脂活性。方法 采用硅胶、Sephadex LH-20、ODS、半制备液相等色谱方法对莱菔子70%乙醇提取物分离纯化，综合应用波谱数据和理化性质鉴定化合物结构。通过3T3-L1前脂肪细胞的胰岛素抵抗模型评价化合物的体外调脂活性。结果 从莱菔子70%乙醇提取物中分离纯化得到了14个化合物，分别鉴定为降莱菔硫代酸乙酯（1）、莱菔酸甲酯（2）、芥子碱（3）、(E)-2-(4-hydroxy-2-oxoindolin-3-ylidene) acetonitrile（4）、2-(4-hydroxy-2-oxoindolin-3-yl) acetonitrile（5）、6-methoxyindole-3-carboxylic acid O-β-D-glucopyranosyl ester（6）、cappariloside A（7）、3',6-二芥子酰基蔗糖（8）、吐叶醇（9）、3,4-二羟基苯甲醛（10）、3,4-二羟基苯甲酸（11）、5-羟甲基糠醛（12）、2-羟甲基-5-呋喃丙烯酸乙酯（13）和β-谷甾醇（14）。结论 化合物1 和2 为新化合物。两者的生源前体均为甲硫氨酸。化合物1 为异硫氰酸酯和乙醇的人工产物。化合物4 、6 、10 、11 、13 为首次从莱菔子中分离得到。体外活性实验结果表明，化合物1 和2 均具有一定的调脂活性。

Objective To study the chemical constituents and lipid regulating activity of Laifuzi (Raphani Semen). Methods The 70% ethanol extract of Raphani Semen was separated and purified by various chromatographic techniques such as silica gel, Sephadex LH-20, ODS and semi-preparative liquid chromatographic columns. The structures of these compounds were identified by spectral data and physicochemical properties. Subsequently, lipid-regulating activity of the compounds was evaluated in vitro by the insulin resistance model of 3T3-L1 preadipocytes. Results A total of 14 compounds were separated and purified from the 70% ethanol extract of Raphani Semen, which were identified as: ethyl norsulforaphanate (1), methyl raphanide (2), sinapine (3), (E)-2-(4-hydroxy-2-oxoindolin-3-ylidene) acetonitrile (4), 2-(4-hydroxy-2-oxoindolin-3-yl)acetonitrile (5), 6-methoxyindole-3- carboxylic acid O-β-D-glucopyranosyl ester (6), cappariloside A (7), 3',6-disinapoyl sucrose (8), vomifoliol (9), 3,4-dihydroxy-benzaldehyde (10), 3,4-dihydroxy-benzoic acid (11), 5-hydroxy-methylfurfural (12), 2-hydroxymethyl-5- furanacrylic acid ethyl ester (13) and β-sitosterol (14). Conclusion Compounds 1 and 2 are new compounds, and their biological precursors are both methionine. Furthermore, compound 1 is an artificial product of isothiocyanate reacting with ethanol. Compounds 4, 6, 10, 11 and 13 were isolated from Raphani Semen for the first time. Compounds 1 and 2 had potential anti-adipogenic effect in vitro.

R284.1

国家重点研发计划项目（2019YFC1711006）