[关键词]
[摘要]
目的 探讨杏贝止咳颗粒对豚鼠感染后咳嗽(post-infectious cough,PIC)的治疗作用。方法 除对照组外,将3~4周龄SPF级雄性Hartley豚鼠采用鼻滴脂多糖、烟熏、辣椒素雾化激发来构建PIC豚鼠模型。造模成功后分为模型组及杏贝止咳颗粒低、中、高剂量(0.93、1.86、3.72 g/kg)组、阿斯美(21.62 mg/kg)组和苏黄止咳胶囊(313.88 mg/kg)组,末次给药后24 h,通过辣椒素雾化诱咳测定咳嗽次数及潜伏期,采用ELISA法检测血清、肺泡灌洗液和肺组织中P物质(substance P,SP)、降钙素相关基因肽(calcitonin gene related peptide,CGRP)、神经肽A(neurokinin A,NKA)、神经肽B(neurokinin B,NKB)、神经生长因子(nerve growth factor,NGF)、前列腺素E2(prostaglandin E2,PGE2)和缓激肽(bradykinin,BK)含量,血液分析仪测定肺泡灌洗液中白细胞分类,并用苏木素-伊红(HE)染色法观察肺组织病理变化。结果 与对照组比较,模型组豚鼠体质量上升缓慢,咳嗽次数明显增多(P<0.001),咳嗽潜伏期明显缩短(P<0.001),中性粒细胞和嗜酸性粒细胞占比显著升高(P<0.01、0.001),血清、肺泡灌洗液和肺组织中SP、CGRP、NKA、NKB、NGF、PGE2和BK含量均显著增高(P<0.05、0.01、0.001),肺组织中炎性细胞浸润明显。与模型组比较,杏贝止咳颗粒能够改善PIC豚鼠体质量增加缓慢趋势,显著减少咳嗽次数(P<0.001),延长咳嗽潜伏期(P<0.001),显著降低白细胞中嗜酸性细胞和中性粒细胞比例(P<0.01、0.001),显著降低豚鼠血清、肺泡灌洗液和肺组织中SP、CGRP、NKA、NKB、NGF、PGE2和BK含量(P<0.05、0.01、0.001),改善肺组织病理变化。结论 杏贝止咳颗粒对PIC豚鼠具有治疗作用。
[Key word]
[Abstract]
Objective To explore the effect of Xingbei Zhike Granules (杏贝止咳颗粒, XBZK) on post-infectious cough (PIC). Methods In addition to control group, 3—4 weeks old SPF male Hartley guinea pigs were used to establish PIC guinea pig model by nasal drops of lipopolysaccharide, smoking and capsaicin atomization. After successful modeling, guinea pigs were divided into model group, Xingbei Zhike Granules low-, medium- and high-dose (0.93, 1.86, 3.72 g/kg) groups, Asmeton (21.62 mg/kg) group and Suhuang Zhike Capsules (苏黄止咳胶囊, 313.88 mg/kg) group. After the last administration, cough frequency and latency were measured by capsaicin atomization. Substance P (SP), calcitonin gene related peptide (CGRP), neuropeptide A (NKA) and neuropeptide B (neuropeptide B, NKB), nerve growth factor (NGF), prostaglandin E2 (PGE2) and bradykinin (BK) levels in serum, alveolar lavage fluid and lung tissue were detected by ELISA. The leukocyte classification in alveolar lavage fluid was determined by blood analyzer. Pathological changes of lung tissue were observed by hematoxylin-eosin (HE) staining. Results Compared with control group, the weight of guinea pigs in model group was slowly increased, the number of coughs was significantly increased (P < 0.001), the latent period of cough was significantly shortened (P < 0.001), the proportions of neutrophils and eosinophils were significantly increased (P < 0.01, 0.001), and the contents of SP, CGRP, NKA, NKB, NGF, PGE2 and BK in serum, alveolar lavage fluid and lung tissue were significantly increased (P < 0.01, 0.001). Hyperplasia and inflammatory cell infiltration in lung tissue were obvious. Compared with model group, Xingbei Zhike Granules improved the slow increase trend of body weight in PIC guinea pigs, significantly reduced the number of coughs (P < 0.001), prolonged the cough incubation period (P < 0.001), significantly reduced the proportions of eosinophils and neutrophils in white blood cells (P < 0.01, 0.001), and significantly reduced the contents of SP, CGRP, NKA, NKB, NGF, PGE2 and BK in serum, alveolar lavage fluid and lung tissue (P < 0.05, 0.01, 0.001), relieved the histopathological changes of lung tissue. Conclusion Xingbei Zhike Granule has therapeutic effect on PIC guinea pigs.
[中图分类号]
R285.5
[基金项目]