目的 研究白芍总苷的主要成分芍药苷、芍药内酯苷对胶原诱导性关节炎（collagen-induced arthritis，CIA）大鼠长期给药后的组织分布。方法 建立超高效液相色谱串联质谱（UPLC-MS/MS）法，测定白芍总苷长期给药后大鼠心、肝、脾、肺、肾、小肠、胸腺、滑膜等组织中芍药苷和芍药内酯苷的分布情况。结果 长期给药后，芍药苷和芍药内酯苷主要分布于小肠和肾，其次为脾、胸腺、滑膜、肝、肺、心。2种成分在CIA大鼠中的组织分布大多低于正常大鼠。结论 揭示了白芍总苷长期给药后的组织分布特征，为探讨白芍总苷治疗类风湿性关节炎的靶器官以及疗效的改进提供了参考。

Objective To study the tissue distribution of paeoniflorin and albiflorin, the main components of total glucosides of paeony, in collagen-induced arthritis (CIA) rats after long-term administration. Methods UPLC-MS/MS method was established to determine the distribution of paeoniflorin and albiflorin in heart, liver, spleen, lung, kidney, small intestine, thymus and synovium of rats after long-term administration. Results After long-term administration, paeoniflorin and albiflorin were mainly distributed in small intestine and kidney, followed by spleen, thymus, synovium, liver, lung and heart. The tissue distribution of the two components in CIA rats were mostly lower than that in normal rats. Conclusion The tissue distribution characteristics of total glucosides of paeony after long-term administration are revealed, which provides a reference for exploring the target organ of total glucosides of paeony in the treatment of rheumatoid arthritis and improving the curative effect.

R285.61

国家自然科学基金面上项目（81773860）；北京市教育委员会科学研究计划项目资助（KM201910025021）